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oai:figshare.com:article/34025941 2026-09-29T17:26:54Z category_4 category_8 category_12 category_13 category_14 category_24 category_272 category_873 category_734 category_61 category_132 category_133 category_134 portal_5 item_type_6 month_year_09_2026

<p>Motif sequence and their width.</p> Fatima Umar (20668763) Rizwana Maqbool (838368) Muhammad Ahsan Khan (12568907) Bushra Sadia (11419814) Biochemistry Microbiology Cell Biology Genetics Molecular Biology Evolutionary Biology Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified Biological Sciences not elsewhere classified Developmental Biology Infectious Diseases Plant Biology Virology regulating cellular responses div >< p chromosome number 1 expression analyses provide synthesize phytic acid plant hormone signaling including phylogenetic analysis growth stage expression genome wide identification silico expression analysis inositol phosphate metabolism triticum aestivum </ hexaploid wheat genome genes revealed treatment motif analysis showed >) gene family plant growth wide analysis expression profiling gene family comprehensive genome aestivum </ phyti acid plant physiology genes revealed synteny analysis promoter analysis taitpk </ itpks </ itpk </ ipk2 </ ipk1 </ vital role three genomes sequences represented preliminary qrt preferential tissue pcr profiling ins134_p3_kin domain functional characterization <div><p>Inositol tetrakisphosphates kinase (<i>ITPK</i>) plays a vital role in regulating cellular responses to abiotic stress, plant hormone signaling and various other aspects of plant growth and development. Additionally, it is also involved in phosphorous, inositol phosphate metabolism and synthesize phytic acid which is the main phosphorous storage form in seeds. While essential for plant physiology, high phytic acid levels reduce the bioavailability of minerals such as Fe and Zn, which impact nutritional quality negatively. Despite its importance, the <i>ITPK</i> gene family remains largely unexplored in bread wheat. In this study, we conducted a comprehensive genome-wide analysis, including phylogenetic analysis and expression profiling, of the <i>ITPK</i> gene family in wheat. We systematically examined gene structure, chromosomal distribution, motif and domain conservation, promoter analysis, synteny analysis, protein modeling and protein-protein interaction of <i>ITPKs</i>. A total of 15 <i>ITPK</i> genes have been identified in wheat (<i>T. aestivum</i>). The <i>TaITPK</i> genes were distributed on chromosome number 1, 4, 5 and 7 in all the three genomes A, B and D. Comparative phylogenetic analysis resolved four major groups, with wheat <i>ITPK</i> sequences represented in Groups I, III, and IV, whereas Group II contained no wheat sequence. The conserved domain and motif analysis showed the presence of Ins134_P3_kin domain at N-terminus and CPase_L_D2 in C-terminus of protein. String based protein interaction analysis suggested <i>ITPK</i> genes interact with Inositol polyphosphate multi kinase (<i>IPK1</i>) and Inositol-pentakisphosphate 2 kinase (<i>IPK2</i>) which were also involve in synthesis of phyti acid. Furthermore, in-silico expression analysis of <i>ITPK</i> genes revealed their preferential tissue-specific and growth stage expression, especially after anthesis of wheat. Preliminary qRT-PCR profiling of selected <i>TaITPK</i> genes revealed treatment-dependent relative expression patterns under FeSO₄ and ZnSO₄ supplementation. The current study has identified 15 <i>ITPK</i> genes present in hexaploid wheat genome. Their evolutionary, structural, and expression analyses provide a foundation for further functional characterization of this gene family in wheat.</p></div> 2026-09-29T17:26:49Z Text Journal contribution 10.1371/journal.pone.0359528.s003 https://figshare.com/articles/journal_contribution/_p_Motif_sequence_and_their_width_p_/34025941 CC BY 4.0