2026-10-11T12:37:37Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34025775 2026-09-29T17:25:27Z category_8 category_12 category_15 category_46 category_64 category_122 category_128 category_132 portal_5 item_type_3 month_year_09_2026

Primer sequences used in qPCR. Hiroki Kono (2321905) Toshihiro Sashihara (3145098) Microbiology Cell Biology Neuroscience Immunology Cancer Mental Health Hematology Infectious Diseases since several reports play critical roles lactic acid bacteria div >< p inhibiting viral infections viral immune responses plasmacytoid dendritic cells lactobacillus delbrueckii </ streptococcus thermophilus </ conventional dendritic cells ols3059 activate cdcs dendritic cells ols3059 induced anti viral antigens cell responses thermophilus </ l </ bulgaricus </ respiratory symptoms recent studies previous studies interferon production findings indicate components responsible also showed <div>Dendritic cells are classified into conventional dendritic cells (cDCs) and plasmacytoid dendritic cells (pDCs), both of which play critical roles in anti-viral immune responses. Previous studies have reported that dairy consumption of yogurt fermented with Lactobacillus delbrueckii subsp. bulgaricus OLL1073R-1 and Streptococcus thermophilus OLS3059 had the effect of inhibiting viral infections. Recent studies have shown that the activation of both cDCs and pDCs and the decrease of respiratory symptoms were induced by the consumption of this yogurt. However, the components responsible for these effects and the underlying mechanisms remain unclear. Since several reports have shown that cDCs and pDCs were activated by lactic acid bacteria, we hypothesized L. bulgaricus OLL1073R-1 and S. thermophilus OLS3059 induced anti-viral immune responses by activating cDCs and pDCs. Here we demonstrated that both L. bulgaricus OLL1073R-1 and S. thermophilus OLS3059 activate cDCs and pDCs in human peripheral blood mononuclear cells. We also showed that both cDCs and pDCs were necessary for type I interferon production, whereas cDCs, but not pDCs, were essential for enhancing effector memory CD4(+) T cell responses to viral antigens when stimulated by L. bulgaricus OLL1073R-1. These findings indicate that anti-viral immune responses induced by consumption of this yogurt are mediated by both L. bulgaricus OLL1073R-1 and S. thermophilus OLS3059 through activation of cDCs and pDCs.</div> 2026-09-29T17:25:10Z Dataset Dataset 10.1371/journal.pone.0359545.s011 https://figshare.com/articles/dataset/_p_Primer_sequences_used_in_qPCR_p_/34025775 CC BY 4.0