2026-10-12T05:07:32Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34025458 2026-09-29T17:22:25Z category_4 category_8 category_12 category_13 category_24 category_272 category_734 portal_5 item_type_6 month_year_09_2026

<p>Rawgel.</p> Andrew E. Maclean (10219517) Orsola Iorillo (22772687) Drahomíra Faktorová (373414) Monika Singh (593097) Suzanne McGill (11916067) Julius Lukeš (122896) Lilach Sheiner (79023) Biochemistry Microbiology Cell Biology Genetics Evolutionary Biology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified shares new components mitoribosome possesses many div >< p highly conserved feature perkinsus marinus </ divergent subunit composition divergent mitochondrial complexes perkinsus </ conserved across toxoplasma </ plasmodium </ mitochondrial complexes metc complexes mitochondrial ribosome mitochondrial function xlink "> wider lineages unexpected diversity transcription factors study reveals outer membrane observed enlarged myzozoan parasite metc ), including members deadly apicomplexan complexome profiling cell survival atp synthase apiap2 family <div><p>Mitochondrial complexes, such as the mitochondrial electron transport chain (mETC), the F₁F_o-ATP synthase, the mitochondrial ribosome and the Translocase of the Outer Membrane (TOM) complex are centrally important for mitochondrial function and cell survival. Recently, an unexpected diversity in the composition of these complexes across unicellular eukaryotic lineages, with the apicomplexan parasites featuring prominently, has been revealed. However, whether the observed enlarged and divergent mitochondrial complexes are conserved across the wider lineages has not been investigated. Here, using a complexome profiling proteomic approach, we have characterised the composition of the mitochondrial complexes of the myzozoan parasite of oysters, <i>Perkinsus marinus</i>. We show that it shares new components of ATP synthase and the mETC complexes that in the deadly apicomplexan <i>Plasmodium</i> and <i>Toxoplasma</i> were shown to be important for growth. Moreover, the <i>Perkinsus</i> mitoribosome possesses many of the divergent features recently discovered in apicomplexans, including members of the ApiAP2 family that until recently were all considered to be transcription factors. Our study reveals that the divergent subunit composition of mitochondrial complexes is an ancestral and highly conserved feature of Myzozoa.</p></div> 2026-09-29T17:22:05Z Text Journal contribution 10.1371/journal.ppat.1014543.s015 https://figshare.com/articles/journal_contribution/_p_Rawgel_p_/34025458 CC BY 4.0