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oai:figshare.com:article/34025419 2026-09-29T17:22:09Z category_4 category_8 category_12 category_13 category_24 category_272 category_734 portal_5 item_type_1 month_year_09_2026

Analysis of mETC complexes and ISPR. Andrew E. Maclean (10219517) Orsola Iorillo (22772687) Drahomíra Faktorová (373414) Monika Singh (593097) Suzanne McGill (11916067) Julius Lukeš (122896) Lilach Sheiner (79023) Biochemistry Microbiology Cell Biology Genetics Evolutionary Biology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified shares new components mitoribosome possesses many div >< p highly conserved feature perkinsus marinus </ divergent subunit composition divergent mitochondrial complexes perkinsus </ conserved across toxoplasma </ plasmodium </ mitochondrial complexes metc complexes mitochondrial ribosome mitochondrial function xlink "> wider lineages unexpected diversity transcription factors study reveals outer membrane observed enlarged myzozoan parasite metc ), including members deadly apicomplexan complexome profiling cell survival atp synthase apiap2 family (A) Heatmaps of the complexome profiles from whole-cell complexome of mETC complex subunits from complex II, complex III and complex IV. Each row represents a gel slice, with the gel slice number indicated on the left and the molecular weight, based on the migration of soluble proteins (blue), on the right. Subunit IDs are shown at the bottom of their corresponding profile, with mitochondrially-encoded subunits highlighted in red. Dark blue indicates the highest relative abundance (1) and white the lowest (0). Coomassie-stained Native PAGE gel on which the analysis was performed is depicted on the left of the profile. (B) Re-analysis of Toxoplasma complex II immunoprecipitation from [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1014543#ppat.1014543.ref013" target="_blank">13</a>], with putative TgSDHC added to the search database. Confirmed Toxoplasma complex II subunits highlighted in blue and putative TgSHDC highlighted in purple. (C) Alignment of ISPR proteins from Perkinsus olensi (ISPR1, A0A7J6TKI1; ISPR2, A0A7J6PI35), Toxoplasma gondii (S7UWH3), Besnoitia besnoiti (A0A2A9M9Y8), Cystoisospora suis (A0A2C6LGZ5), Eimeria brunetti (U6LJF9) and Cyclospora cayetanensis (A0A1D3D252). Alignments were made using Clustal Omega and visualised using JalView (v10.0.7) [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1014543#ppat.1014543.ref099" target="_blank">99</a>]. (D) Structural predictions, generated using Alphafold3 [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1014543#ppat.1014543.ref100" target="_blank">100</a>], of P. olensi ISPR1 (pTM score 0.65) (left) and T. gondii ISPR (pTM score 0.69) (middle), coloured according to pIDDT score, as well as superimposition of the two structures (right; PoISPR1 in blue, TgISPR in purple). (RMSD = 0.73 Å between pruned atom pairs). Visualised using ChimeraX [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1014543#ppat.1014543.ref092" target="_blank">92</a>]. (E) Transcript levels of TGME49_224932 in tachyzoites and bradyzoites from [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1014543#ppat.1014543.ref042" target="_blank">42</a>] and [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1014543#ppat.1014543.ref043" target="_blank">43</a>]. (F) Immunoblot analysis of Toxoplasma gondii expressing a Ty-epitope tagged version of TgISPR using the anti-Ty antibody. (TIF) 2026-09-29T17:22:05Z Image Figure 10.1371/journal.ppat.1014543.s002 https://figshare.com/articles/figure/_p_Analysis_of_mETC_complexes_and_ISPR_p_/34025419 CC BY 4.0