2026-10-14T20:02:59Z
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2026-09-29T17:22:07Z
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<p>Generating complexome profiles.</p>
Andrew E. Maclean (10219517)
Orsola Iorillo (22772687)
Drahomíra Faktorová (373414)
Monika Singh (593097)
Suzanne McGill (11916067)
Julius Lukeš (122896)
Lilach Sheiner (79023)
Biochemistry
Microbiology
Cell Biology
Genetics
Evolutionary Biology
Environmental Sciences not elsewhere classified
Biological Sciences not elsewhere classified
shares new components
mitoribosome possesses many
div >< p
highly conserved feature
perkinsus marinus </
divergent subunit composition
divergent mitochondrial complexes
perkinsus </
conserved across
toxoplasma </
plasmodium </
mitochondrial complexes
metc complexes
mitochondrial ribosome
mitochondrial function
xlink ">
wider lineages
unexpected diversity
transcription factors
study reveals
outer membrane
observed enlarged
myzozoan parasite
metc ),
including members
deadly apicomplexan
complexome profiling
cell survival
atp synthase
apiap2 family
<p>(A) Coomassie-stained Native PAGE gels showing molecular weight markers (i- NativeMark, ii- Bovine mitochondrial membranes), <i>Perkinsus</i> mitochondrially-enriched material (iii) and <i>Perkinsus</i> whole-cell material (iv) used in generating the complexome. Molecular weight markers and <i>Perkinsus</i> material were run on the same gel and aligned, with corresponding gel slices numbers (1–48/60) shown on the right-hand side. For the mitochondrially-enriched material, gels from both replicate 1 and 2 are shown. (B) Comparison of proteins identified in each repetition of the mitochondrial and the whole-cell samples. (C) Mass calibration analysis using NativeMark and Bovine mitochondrial membrane molecular weight markers. Molecular weight and corresponding gel slice were plotted and a non-linear regression, semi-log line plotted. Full details of mass calibration are in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1014543#ppat.1014543.s008" target="_blank">S3 Table</a>.</p> <p>(TIF)</p>
2026-09-29T17:22:05Z
Image
Figure
10.1371/journal.ppat.1014543.s001
https://figshare.com/articles/figure/_p_Generating_complexome_profiles_p_/34025416
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