2026-10-10T08:18:02Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34025103 2026-09-29T16:22:58Z category_575 portal_316 item_type_1 month_year_09_2026

Image 4_Aptamer-based surface plasmon resonance sensor for α-amanitin.tif Qian Li (114642) Lei Lei (85503) Sha Sha (558995) Food Packaging, Preservation and Safety aptamer food safety sensor surface plasmon resonance α-amanitin <p>α-Amanitin is a highly toxic amatoxin associated with poisonous mushroom poisoning and represents a significant threat to food safety and public health. The complexity and time requirements of conventional instrumental methods highlight the need for rapid and sensitive screening strategies. Herein, we developed a label-free, direct, and regenerable aptamer-based surface plasmon resonance (SPR) biosensor for the detection of α-amanitin. Three previously reported aptamers were systematically screened under identical SPR conditions, and Apt41-BT was selected as the optimal recognition element based on its superior experimental response. The selected aptamer was immobilized on a streptavidin-modified sensor chip through a biotin–streptavidin interaction, enabling real-time monitoring of α-amanitin binding without the need for additional labeling or amplification. The resulting biosensor exhibited a concentration-dependent response over the range of 5–500 nM, with a practical detection limit of 5 nM. Importantly, the sensor surface could be regenerated for repeated measurements while maintaining stable performance. The applicability of the proposed platform was further demonstrated using spiked mushroom samples, which showed satisfactory recoveries and good agreement with LC–MS results. This work establishes an experimentally screened aptamer–SPR sensing strategy that integrates direct detection, sensor regeneration, and mushroom-matrix validation, providing a rapid and potentially field-deployable approach for α-amanitin screening.</p> 2026-09-29T16:22:58Z Image Figure 10.3389/fsufs.2026.1946446.s001 https://figshare.com/articles/figure/Image_4_Aptamer-based_surface_plasmon_resonance_sensor_for_-amanitin_tif/34025103 CC BY 4.0