2026-10-14T13:11:58Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34024950 2026-09-29T16:00:31Z category_37476 portal_1023 item_type_12 month_year_09_2026

From OpenSPIM to FlowSPIM: Enhancing the versatility of a light sheet microscope through an iterative design process Endre Joachim Lerheim Mossige (25137753) Sergey Ponomartsev (726436) Natalia Smirnova (25137756) Wietske van der Ent (185651) Siri Andresen (22232252) Xian Hu (191432) Felix Margadant (191422) Rainer Heintzmann (112278) Roland Kádár (25137759) Kesavan Sekar (25137762) Helene Knævelsrud (3604124) Camila Vicencio Esguerra (25137765) Stefan Krauss (217340) Dag Kristian Dysthe (13805792) Alexander Refsum Jensenius (4277257) Physics (arXiv) physics.optics (arXiv) cond-mat.soft (arXiv) Light sheet microscopy is ideal for the 3D imaging of large biological specimens, yet systematic documentation on adapting microscope designs to diverse experimental requirements remains limited. We present the iterative process used to design and build a modular light-sheet microscope and adapt it to different sample types and experimental conditions. Starting from a stripped-down OpenSPIM platform, we gradually make our microscope more versatile by implementing new hardware, such as additional laser channels and optical filters, and new protocols for sample handling. In its final iteration, FlowSPIM is a new flow-based incubation technique for 3D tissue culture. 2026-09-25T00:00:00Z Text Preprint 10.48550/arXiv.2609.31115 https://figshare.com/articles/preprint/From_OpenSPIM_to_FlowSPIM_Enhancing_the_versatility_of_a_light_sheet_microscope_through_an_iterative_design_process/34024950 CC0