2026-10-14T05:50:09Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34024668 2026-09-29T14:48:22Z category_1 category_4 category_7 category_12 category_13 category_14 category_16 category_873 category_46 category_61 category_64 category_128 category_135 portal_63 item_type_6 month_year_09_2026

Glycitin Targets the Orphan Receptor GPR3 to Promote Milk Fat Synthesis in Goat Mammary Epithelial Cells Liaoyu Fan (25137567) Chu Chen (102135) Xiaoran Li (656489) Runyu Yang (17257291) Hua Wang (13530) Huaiping Shi (3643912) Biophysics Biochemistry Medicine Cell Biology Genetics Molecular Biology Physiology Chemical Sciences not elsewhere classified Immunology Developmental Biology Cancer Hematology Computational Biology molecular dynamics suggested lipid droplet formation induced lipid synthesis hydroxy fatty acids fatty acid esters dairy product quality cyclic adenosine monophosphate coupled receptor 3 associated factor x glycitin also increased findings identify gpr3 orphan receptor gpr3 lipogenic gene expression knockdown reduced triglycerides receptor mechanisms reduced myc associated signaling gpr3 </ gpr3 ). glycitin targets examined glycitin major determinant g protein fahfas ). Milk fat is a major determinant of dairy product quality, but the receptor mechanisms by which dietary isoflavones regulate mammary lipid metabolism remain unclear. We examined glycitin-induced lipid synthesis in goat mammary epithelial cells (GMECs) and the involvement of G protein-coupled receptor 3 (GPR3). Glycitin increased triglyceride accumulation, lipid droplet formation, and lipogenic gene expression. Docking and molecular dynamics suggested a plausible glycitin–GPR3 interaction, while the increase in cyclic adenosine monophosphate (cAMP) was weakened by <i>GPR3</i> knockdown. Glycitin also increased <i>GPR3</i> expression and reduced MYC-associated factor X (MAX)-associated transcriptional repression. <i>GPR3</i> knockdown reduced triglycerides, diacylglycerols, and fatty acid esters of hydroxy fatty acids (FAHFAs). These findings identify GPR3-associated signaling as a potential mechanism linking glycitin to enhanced milk fat synthesis in GMECs. 2026-09-29T00:00:00Z Text Journal contribution 10.1021/acs.jafc.6c00383.s001 https://figshare.com/articles/journal_contribution/Glycitin_Targets_the_Orphan_Receptor_GPR3_to_Promote_Milk_Fat_Synthesis_in_Goat_Mammary_Epithelial_Cells/34024668 CC BY-NC 4.0