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oai:figshare.com:article/34024059 2026-09-29T13:38:34Z category_4 category_7 category_12 category_14 category_16 category_873 category_61 category_64 portal_63 item_type_6 month_year_09_2026

Probiotic-Derived Exopolysaccharides Enhance Mesenchymal Stem Cell-Mediated Fibroblast Repair under Hyperglycemic Conditions Mesude Bicer (22279266) Mert Akdur (25137270) Esra Battal (25137273) Fatma Sener (22279272) Irem Yilmaz (25137276) Özkan Fidan (22279278) Biochemistry Medicine Cell Biology Molecular Biology Physiology Chemical Sciences not elsewhere classified Developmental Biology Cancer supportive bioactive agent preliminarily characterized prior persistent oxidative stress oxidative stress levels mesenchymal stem cells mediated fibroblast repair improve tissue repair delayed tissue regeneration decreased fibroblast activity propidium iodide staining apoptotic cell death glucose conditions prior diabetic wound healing wound closure healing wounds diabetic microenvironment da staining subsequent cell cell viability like conditions hyperglycemic conditions strongest effect scratch assay relatively weaker regenerative responses potential agents plantarum </ often lead mtt assay migration potential lactobacillus plantarum lactobacillus brevis l929 fibroblasts hyperglycemic microenvironment findings suggest examined whether eps fractions eps derived derived eps derived biomolecules conditioned medium closely associated chronic non brevis </ based experiments bacterial exopolysaccharides attracted attention assessed using Diabetic wounds are closely associated with persistent oxidative stress, decreased fibroblast activity, and delayed tissue regeneration under hyperglycemic conditions. These pathological features particularly complicate wound healing and often lead to chronic non-healing wounds. Recently, mesenchymal stem cells (MSCs) and probiotic-derived biomolecules have attracted attention as potential agents to improve tissue repair. In this study, we examined whether the combination of adipose-derived mesenchymal stem cell-conditioned medium (ADMSC-CM) and bacterial exopolysaccharides (EPSs) could improve fibroblast responses under diabetes-like conditions. EPSs were extracted from Lactobacillus plantarum and Lactobacillus brevis, purified and preliminarily characterized prior to subsequent cell-based experiments. To stimulate a diabetic microenvironment, L929 fibroblasts were exposed to high-glucose conditions prior to treatment with ADMSC-CM, purified EPSs, or their combination. Cell viability, oxidative stress levels, apoptosis, and migration potential were assessed using the MTT assay, DCFH-DA staining, acridine orange/propidium iodide staining, and scratch assay, respectively. Interestingly, the combination of ADMSC-CM with <i>L. brevis</i>-derived EPS showed the strongest effect. This treatment improved fibroblast viability and wound closure under hyperglycemic conditions while significantly reducing intracellular ROS levels and apoptotic cell death. EPS derived from <i>L. plantarum</i> also showed supportive effects, but these regenerative responses were relatively weaker. Both EPS fractions were found to be biocompatible at 1000 μg/mL. Overall, these findings suggest that probiotic-derived EPS can enhance ADMSC-mediated fibroblast repair in a hyperglycemic microenvironment and serve as a supportive bioactive agent in diabetic wound healing. 2026-09-29T00:00:00Z Text Journal contribution 10.1021/acsomega.6c07182.s001 https://figshare.com/articles/journal_contribution/Probiotic-Derived_Exopolysaccharides_Enhance_Mesenchymal_Stem_Cell-Mediated_Fibroblast_Repair_under_Hyperglycemic_Conditions/34024059 CC BY-NC 4.0