2026-10-14T08:22:11Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34023674 2026-09-29T12:11:28Z category_4 category_8 category_12 category_14 category_21 category_46 category_734 category_61 portal_87 item_type_3 month_year_09_2026

Integrative multi-omics analysis of network pharmacology reveals the active components and mechanisms of <i>Olibanun</i> in improving atopic dermatitis Xiaobi Zhang (25136990) Pengyuan Du (17507646) Wenqi Tang (4675531) Yinling Zhou (25136993) Jing Cao (287196) Lusheng Wang (513360) Jianli Pan (25136996) Biochemistry Microbiology Cell Biology Molecular Biology Biotechnology Immunology Biological Sciences not elsewhere classified Developmental Biology Olibanun atopic dermatitis network pharmacology single-cell sequencing g immune microenvironment keratinocyte-T-cell communication axis <p>This study investigates potential active ingredients of Olibanun in ameliorating atopic dermatitis (AD) and underlying mechanisms. Active components were screened via TCMSP. Anti-AD targets predicted using multiple databases PPI networks were built with STRING/Cytoscape. GO/KEGG enrichment performed with R. Molecular docking used AutoDock. RNA-seq analyzed effects on HaCaT cells. TNF-α/IFN-γ-induced AD models evaluated anti-inflammatory effects. DNCB-induced AD mice tested <i>Olibanun</i> extract (OE) ointment protection. ScRNA-seq analyzed AD skin heterogeneity. Thirteen active components and 38 anti-AD targets were identified, mainly regulate inflammation and immune-related pathways. Docking confirmed strong binding to IKBKB. <i>In vitro,</i> active components exerts anti-inflammatory effects via NF-κB. OE ointment alleviated skin inflammation and decreased inflammatory cytokines. ScRNA-seq revealed keratinocyte-T cell axis dysregulation and IL-17/NF-κB signalling activation in AD skin. Olibanun may regulate the IL-17/NF-κB pathway in keratinocytes and modulate keratinocyte-T cell communication linked to AD pathology.</p> 2026-09-29T12:11:28Z Dataset Dataset 10.6084/m9.figshare.34023674.v1 https://figshare.com/articles/dataset/Integrative_multi-omics_analysis_of_network_pharmacology_reveals_the_active_components_and_mechanisms_of_i_Olibanun_i_in_improving_atopic_dermatitis/34023674 CC BY 4.0