2026-10-06T19:01:58Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34023546 2026-09-29T11:57:34Z category_398 category_399 category_400 category_401 category_402 category_19 category_403 category_404 portal_316 item_type_1 month_year_09_2026

Image_1_A Penicillin Derivative Exerts an Anti-Metastatic Activity in Melanoma Cells Through the Downregulation of Integrin αvβ3 and Wnt/β-Catenin Pathway.tif Elizabeth Barrionuevo (8488278) Florencia Cayrol (8488281) Graciela A. Cremaschi (8488284) Patricia G. Cornier (5798657) Dora B. Boggián (8488287) Carina M. L. Delpiccolo (8488290) Ernesto G. Mata (1581094) Leonor P. Roguin (8488293) Viviana C. Blank (8488296) Basic Pharmacology Clinical Pharmacology and Therapeutics Clinical Pharmacy and Pharmacy Practice Pharmaceutical Sciences Pharmacogenomics Pharmacology Toxicology (incl. Clinical Toxicology) Pharmacology and Pharmaceutical Sciences not elsewhere classified triazolylpeptidyl penicillin anti-metastatic effect murine melanoma β-catenin metalloproteinases-2 and -9 integrin αvβ3 <p>The synthetic triazolylpeptidyl penicillin derivative, named TAP7f, has been previously characterized as an effective antitumor agent in vitro and in vivo against B16-F0 melanoma cells. In this study, we investigated the anti-metastatic potential of this compound on highly metastatic murine B16-F10 and human A375 melanoma cells. We found that TAP7f inhibited cell adhesion, migration and invasion in a dose-dependent manner. Additionally, we demonstrated that TAP7f downregulated integrin αvβ3 expression and Wnt/β-catenin pathway, a signaling cascade commonly related to tumor invasion and metastasis. Thus, TAP7f reduced both the enzymatic activity and the expression levels of matrix-metalloproteinases-2 and -9 in a time dependent manner. Moreover, TAP7f inhibited the expression of the transcription factor Snail and the mesenchymal markers vimentin, and N-cadherin, and up-regulated the expression of the epithelial marker E-cadherin, suggesting that the penicillin derivative affects epithelial–mesenchymal transition. Results obtained in vitro were supported by those obtained in a B16-F10-bearing mice metastatic model, that showed a significant TAP7f inhibition of lung metastasis. These findings suggest the potential of TAP7f as a chemotherapeutic agent for the treatment of metastatic melanoma.</p> 2026-09-29T11:57:34Z Image Figure 10.3389/fphar.2020.00127.s001 https://figshare.com/articles/figure/Image_1_A_Penicillin_Derivative_Exerts_an_Anti-Metastatic_Activity_in_Melanoma_Cells_Through_the_Downregulation_of_Integrin_v_3_and_Wnt_-Catenin_Pathway_tif/34023546 CC BY 4.0