2026-10-06T08:12:30Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34022555 2026-09-29T10:08:17Z category_4 category_915 category_8 category_12 category_14 category_16 category_19 category_21 category_272 category_873 category_69 category_128 portal_63 item_type_6 month_year_09_2026

4‑Biphenylboronic Acid Exerts Antifungal Activity against Neopestalotiopsis ellipsospora via Dual Targeting of Plasma Membrane Integrity and Cell Wall Biogenesis Shuangqing Tan (25136528) Hongfei Gao (4907677) Jianmei Yao (8405460) Huifang Liu (792239) Lulu Li (608444) Rongxiu Yin (25136531) Yao Chen (149006) Zhiwei Lei (1755997) Wen Yang (235745) Biochemistry Physical Sciences not elsewhere classified Microbiology Cell Biology Molecular Biology Physiology Pharmacology Biotechnology Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified Inorganic Chemistry Hematology processed tea quality mitochondrial energy metabolism cell wall biogenesis plasma membrane integrity impaired membrane bioenergetics mechanistic analyses indicated chitin synthase 1 50 </ sub membrane function enzymatic analyses chitin biosynthesis transcriptome profiling study investigated solute transport rna sequencing reducing yield identified downregulation genes associated findings suggest electron microscopy economic losses coupled receptor coordinated disruption antifungal activity alkaline ceramidase 72 mg Neopestalotiopsis ellipsospora causes tea leaf spots, reducing yield and processed tea quality and resulting in economic losses. This study investigated the antifungal activity and mechanism of 4-biphenylboronic acid using mycelial growth inhibition assays, enzymatic analyses, electron microscopy, and RNA sequencing. The compound showed strong antifungal activity against N. ellipsospora with an EC₅₀ of 10.72 mg/L. Mechanistic analyses indicated that 4-biphenylboronic acid disrupted plasma membrane integrity, impaired membrane bioenergetics and solute transport, and perturbed cell wall homeostasis. Transcriptome profiling further identified downregulation of genes associated with chitin biosynthesis, including plasma membrane H⁺-ATPase, alkaline ceramidase, G-protein-coupled receptor, and chitin synthase 1. Together, these findings suggest that 4-biphenylboronic acid inhibits N. ellipsospora through the coordinated disruption of mitochondrial energy metabolism, membrane function, and cell wall biogenesis. 2026-09-29T00:00:00Z Text Journal contribution 10.1021/acs.jafc.6c12098.s001 https://figshare.com/articles/journal_contribution/4_Biphenylboronic_Acid_Exerts_Antifungal_Activity_against_Neopestalotiopsis_ellipsospora_via_Dual_Targeting_of_Plasma_Membrane_Integrity_and_Cell_Wall_Biogenesis/34022555 CC BY-NC 4.0