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Cellulose
Reshapes Microbial C–S–Fe
Coupling to Suppress Direct and Fe(III)-Mediated Pyrite Oxidation
Min Zhang (111999)
Yu Liu (6938)
Guoping Jiang (401393)
Jing Wen (25059)
Ling Xia (43628)
Ibrahim Ahmed Ibrahim (25136486)
Fenliang Fan (600583)
Zhenghua Liu (458518)
Xueduan Liu (519039)
Qingyun Yan (188165)
Delong Meng (381224)
Huaqun Yin (519037)
Biophysics
Microbiology
Evolutionary Biology
Environmental Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
Ecology
Biological Sciences not elsewhere classified
structural carbon suppresses
process remains unresolved
derived carbon entered
bearing mine soils
assembled genomes collocated
apparent kinetic modeling
amended soil microcosm
sip ), genome
cellulose decreased naoh
– fe coupling
hydrolytic cellulose deconstruction
assimilatory sulfate reduction
sip showed
assimilatory transformations
sulfate uptake
extractable sulfate
whereas time
surface coating
suppress direct
results identify
resolved analysis
reduction genes
geochemical patterns
geochemical measurements
enriched metagenome
effective reactivity
day pyrite
Pyrite oxidation generates sulfate
and acidity in sulfide-bearing
mine soils, but how structural carbon suppresses this process remains
unresolved. We combined a 56-day pyrite-amended soil microcosm with
geochemical measurements, qPCR, metagenomics, DNA stable isotope probing
(DNA-SIP), genome-resolved analysis, and apparent kinetic modeling.
Cellulose decreased NaOH-extractable sulfate by 72.71% and HCl-extractable
Fe(III) by 55.13% at day 56. DNA-SIP showed that cellulose-derived
carbon entered a phylogenetically distributed assimilating guild with
genomic potential for oxidative-hydrolytic cellulose deconstruction,
Fe(III) reduction, sulfate uptake, and assimilatory sulfate reduction.
Enriched metagenome-assembled genomes collocated these C, Fe, and
S functions within individual populations, whereas time-series metagenomes
showed sustained cellulose-deconstruction and sulfur-assimilation
potential without broad enrichment of Fe-reduction genes. Thus, functionally
important assimilators could reshape Fe–S cycling without consistently
dominating bulk community abundance. Consistent with these genomic
and geochemical patterns, the apparent model indicated weaker O<sub>2</sub>-driven direct oxidation, Fe(II) oxidation/Fe(III) regeneration,
and effective reactivity of extractable Fe(III). These results identify
a microbial mechanism whereby cellulose utilization redirects C–S–Fe
coupling toward more reductive and assimilatory transformations, suppressing
pyrite oxidation without detectable mineral-surface coating.
2026-09-29T00:00:00Z
Text
Journal contribution
10.1021/acs.est.5c14280.s001
https://figshare.com/articles/journal_contribution/Cellulose_Reshapes_Microbial_C_S_Fe_Coupling_to_Suppress_Direct_and_Fe_III_-Mediated_Pyrite_Oxidation/34022543
CC BY-NC 4.0