2026-10-13T06:27:36Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34021997 2026-09-29T08:05:37Z category_4 category_7 category_13 category_14 category_16 category_19 category_873 portal_63 item_type_6 month_year_09_2026

Engineering a Galactosylated Zein-Orientin Nanoplatform for NAFLD Alleviation via Lipid Metabolic Modulation and Gut Microbiota Remodeling Yinger Lan (25136024) Guanhong Jin (25136027) Xiaojuan Lei (2831318) Jichun Zhao (120536) Lin Lei (400470) Yalin Ran (19232458) Jian Ming (12902948) Biochemistry Medicine Genetics Molecular Biology Physiology Pharmacology Chemical Sciences not elsewhere classified uniform spherical nanoparticles total cholesterol accumulation high loading capacity existing pharmacological alternatives exhibiting comparable efficacy decreasing pseudomonadota proliferation considerable side effects attenuated lipid peroxidation 89 ± 0 3 ± 2 mediated protein nanoplatform restored antioxidant capacity galactosylated zein nanoplatform galactosylated zein orientin nanoplatform probable toxicity poor solubility phosphatidylethanolamine ratio natural flavonoid maillard reaction low bioavailability hepatoprotective potential gastrointestinal stability engineered via ceramide levels bacteroidetes ratio akkermansia </ 6 nm 14 %) Nonalcoholic fatty liver disease (NAFLD) is characterized by excessive hepatic lipid accumulation, and the existing pharmacological alternatives are constrained by considerable side effects and probable toxicity. Orientin (Ori), a natural flavonoid with hepatoprotective potential, is restricted by poor solubility and low bioavailability. Herein, a galactosylated zein nanoplatform (Ori@Gal-Zein) was engineered via the Maillard reaction, resulting in uniform spherical nanoparticles (163.3 ± 2.6 nm) with high loading capacity of Ori (7.89 ± 0.14%) and gastrointestinal stability. In a CDAHFD-induced NAFLD mouse model, Ori@Gal-Zein decreased hepatic triglyceride and total cholesterol accumulation, attenuated lipid peroxidation, and restored antioxidant capacity, exhibiting comparable efficacy to silybin. Moreover, lipidomics revealed decreased triglyceride, diglyceride, and ceramide levels and restored the phosphatidylcholine/phosphatidylethanolamine ratio. Importantly, Ori@Gal-Zein remodeled gut microbiota by normalizing the Firmicutes/Bacteroidetes ratio, decreasing Pseudomonadota proliferation, and increasing <i>Akkermansia</i> abundance to alleviate NAFLD, providing a selection for carbohydrate-mediated protein nanoplatform. 2026-09-29T00:00:00Z Text Journal contribution 10.1021/acs.jafc.6c06959.s001 https://figshare.com/articles/journal_contribution/Engineering_a_Galactosylated_Zein-Orientin_Nanoplatform_for_NAFLD_Alleviation_via_Lipid_Metabolic_Modulation_and_Gut_Microbiota_Remodeling/34021997 CC BY-NC 4.0