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oai:figshare.com:article/34021386 2026-09-29T05:27:32Z category_34 portal_316 item_type_3 month_year_09_2026

Table 1_Long-term anaerobic biodegradation of miocene lignite by indigenous microbial communities: insights into carbon and nitrogen cycling.docx Michał Bucha (5127470) Piotr Siupka (25135437) Anna Detman-Ignatowska (25135440) Sushmita Deb (21739085) Agnieszka Bylina (25135443) Daria Kaim (25135446) Aleksandra Chojnacka (186642) Dominika Kufka (25135449) Dominika Lewicka-Szczebak (4032590) Anna Sikora (465861) Leszek Marynowski (2443345) Environmental Science anaerobic biodegradation lignite microbial community from coal molecular composition N2-fixation near-surface organic matter cycling rhizobiales <p>To better understand processes in coal environment Miocene detritic lignite from the opencast mine was incubated under anaerobic conditions in the dark (headspace atmosphere 85% N₂, 10% CO₂, 5% H₂) for 3 years. The natural microbial community of detritic lignite was stimulated for growth through the addition of trace elements, vitamins, and carbon-bearing compounds such as yeast extract, nutrient broth, methanol, and sodium acetate. A visual indicator of microbial activity was observed as the color of the fermentation solutions changed over time: from colourless to light yellow (after 3 months), dark brown (after 6 months), and finally black (after 1 year). This progression suggests the dissolution of fulvic and humic acids. At the end of the cultivation, the total nitrogen and total inorganic nitrogen (TIN) contents in the solutions were significantly reduced whereas in incubations with sodium acetate, dissolved organic nitrogen (DON) content significantly increased compared to the initial levels. In most cases, total carbon content significantly increased due to biodegradation, except for the incubations where methanol was added. A GC-MS analysis of the total extracts from lignite revealed that the main macromolecule decomposed by microorganisms was lignin, along with its diagenetic derivatives. The biogas released during the process contained CO₂ and trace amounts of CH₄ (up to 50 ppm). Isotopic data indicated the occurrence of possible anaerobic CH₄ oxidation. Notably, 16S rRNA gene sequencing identified taxa affiliated with lineages that include known diazotrophs in all investigated samples, members of the order Rhizobiales (families Beijerinckiaceae, Rhizobiaceae).</p> 2026-09-29T05:27:32Z Dataset Dataset 10.3389/fenvs.2026.1903570.s002 https://figshare.com/articles/dataset/Table_1_Long-term_anaerobic_biodegradation_of_miocene_lignite_by_indigenous_microbial_communities_insights_into_carbon_and_nitrogen_cycling_docx/34021386 CC BY 4.0