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oai:figshare.com:article/34021027 2026-09-29T03:51:23Z category_4 category_146 category_7 category_12 category_16 category_19 category_21 category_46 category_734 category_64 portal_81 item_type_1 month_year_09_2026

Additional file 3 of The Tie-2 agonist PMC-403 inhibits endothelial damage induced by e-cigarette vapor or LPS Thorben Pape (10181748) Oleg Pak (5748923) Theresa Graalmann (8407923) Christine Ehlers (25135000) Janik Deutscher (25135003) David Spiegelberg (25135006) Paul Horn (297980) Klaus Stahl (5907686) Marius M. Hoeper (9315867) Sascha David (319986) Natascha Sommer (2586913) Benjamin Seeliger (6943853) Biochemistry Space Science Medicine Cell Biology Physiology Pharmacology Biotechnology Immunology Biological Sciences not elsewhere classified Cancer Systemic inflammation ARDS Capillary leakage ECVE ECV Supplementary Material 3: Figure S2: The effect of the Tie2 agonist PMC-403 on the Angpt/Tie2 system in HUVECs. (A) HUVECs were stimulated with different concentrations of the Tie2 agonist PMC-403 for 24 hours. The Angpt-2 concentrations in the supernatant were measured by ELISA. Each group consisted of n=3. Data was analyzed by One-Way ANOVA with according post-hoc tests. (B) Additionally, Angpt-2 transcription was measured by qPCR after stimulation of HUVECs with PMC-403 (n=2) or control (n=3) for 8 hours and analyzed using an unpaired t-test. (C) HUVECs were incubated with 10 µg/ml PMC-403 for 1 hour and then stimulated with LPS. TER was measured by ECIS over time. Each group consisted of n=4. A linear mixed-effects model was used to analyze group and time differences. Results are shown as dot plots with mean ± SD. P-values < 0.05 are considered significant. Abbreviations: Angpt-2 - Angiopoietin-2, HUVECs – human umbilical vein endothelial cells, SD – standard deviation. 2026-09-26T05:00:00Z Image Figure 10.6084/m9.figshare.34021027.v1 https://figshare.com/articles/figure/Additional_file_3_of_The_Tie-2_agonist_PMC-403_inhibits_endothelial_damage_induced_by_e-cigarette_vapor_or_LPS/34021027 CC BY