2026-10-14T03:00:09Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34020660 2026-09-29T03:14:50Z category_4 category_7 category_15 category_16 category_19 category_873 category_734 portal_87 item_type_6 month_year_09_2026

Sinapic acid-mediated green synthesis of silver nanoparticles: characterization, antioxidant capacity and antidiabetic enzyme inhibition Nadia Murshed Abed (25134288) Emel Ekinci (11634867) Zeyad Adil Hameed Hameed (25134291) Şevki Adem (10022395) Biochemistry Medicine Neuroscience Physiology Pharmacology Chemical Sciences not elsewhere classified Biological Sciences not elsewhere classified Silver nanoparticles sinapic acid green synthesis antioxidant activity α-amylase inhibition α-glucosidase inhibition Green synthesis using chemically defined natural phenolic compounds offers a reproducible route to biofunctional nanomaterials. Here, sinapic acid-mediated silver nanoparticles (Sin-AgNPs) were prepared and evaluated for physicochemical characteristics, antioxidant capacity, and inhibition of carbohydrate-hydrolyzing enzymes. Condition screening selected pH 10, 50 °C, a 1:1 sinapic acid/silver nitrate volume ratio, and 8 mM silver nitrate. Ultraviolet–visible spectroscopy showed a surface plasmon resonance band at 400–450 nm. Scanning electron microscopy indicated predominantly spherical particles with dry diameters of approximately 31–96 nm, whereas dynamic light scattering gave a Z-average hydrodynamic diameter of approximately 96–97 nm. The zeta potential was −30.9 mV. Fourier transform infrared spectroscopy detected oxygen-containing bands consistent with sinapic acid-derived species associated with the recovered material, although these data alone do not prove a capping mechanism. In cell-free assays, Sin-AgNPs inhibited α-amylase and α-glucosidase, with IC50 values of 2.01 ± 0.17 and 5.89 ± 0.43 µg/mL, respectively. Under the same assay conditions, acarbose gave IC50 values of 18.55 ± 1.40 and 253.40 ± 5.14 µg/mL, respectively. Sin-AgNPs also produced measurable responses in 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferrous-ion-chelation assays. Because residual free sinapic acid was not quantified, silver nitrate and free sinapic acid were not tested as direct controls, and cytotoxicity, long-term stability, and in vivo efficacy were not evaluated, the results support only preliminary antioxidant and diabetes-related enzyme-inhibition activity and warrant further controlled investigation. 2026-09-29T03:14:50Z Text Journal contribution 10.6084/m9.figshare.34020660.v1 https://figshare.com/articles/journal_contribution/Sinapic_acid-mediated_green_synthesis_of_silver_nanoparticles_characterization_antioxidant_capacity_and_antidiabetic_enzyme_inhibition/34020660 CC BY 4.0