2026-10-13T17:59:42Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34018704 2026-09-29T00:05:36Z category_1 category_4 category_146 category_915 category_7 category_19 category_21 category_272 category_873 portal_63 item_type_6 month_year_09_2026

Preparation of Uniform Nonspherical PLGA Particles in the Nanosize Range by Using a Two-Step Premix Membrane Emulsification Technique Zuyue Liao (14267819) Liang Liu (50022) Yinhui Zhang (773422) Mengyao Xu (6987167) Xinyue Wang (286629) Qiao Hu (297080) Haoran Liu (3940109) Bimin Feng (9567089) Jie Wu (10038) Guojun Wang (729549) Qingze Fan (4521466) Biophysics Biochemistry Space Science Physical Sciences not elsewhere classified Medicine Pharmacology Biotechnology Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified polymeric particulate systems orthogonal experimental design optimization employing one oil phase volume key formulation factors based methods due >- glycolic acid 11 ± 85 offering promising applications classical chemotherapeutic drug advanced drug delivery uniform nonspherical poly including plga concentration uniform nanorods promising candidates nonspherical nanoparticles drug carriers vivo </ vitro </ vinyl alcohol usually produced tumor efficacy systematically evaluated successfully prepared successfully encapsulated spontaneous minimization size range previous work present study optimal conditions nanosize range minor axes methodology provides lactic -< interfacial tension inherent conflict facile route emulsion deformation droplet size deformation inducer critical determinant conventional emulsion co </ buffered saline anisotropic morphology 90 nm The shape of polymeric particulate systems is a critical determinant of their <i>in vivo</i> fate, and nonspherical nanoparticles are promising candidates for advanced drug delivery. However, spherical-shaped particles are usually produced by conventional emulsion-based methods due to the spontaneous minimization of interfacial tension. In our previous work, uniform nonspherical poly(lactic-<i>co</i>-glycolic acid) (PLGA) particles in the micro-size range were successfully prepared by a premix membrane emulsification technique with phosphate-buffered saline (PBS) as a deformation inducer. However, it is difficult to produce nanosized nonspherical particles because of the inherent conflict between the reduction of droplet size and emulsion deformation. To overcome this limitation, a two-step premix membrane emulsification strategy was developed in the present study to temporally and spatially decouple emulsion formation from shape deformation. Through optimization employing one-variable-at-a-time (OVAT) and orthogonal experimental design, the influence of key formulation factors, including PLGA concentration, poly(vinyl alcohol) (PVA) concentration, PBS concentration, and oil phase volume, on the shape of the particles was systematically evaluated. Under the optimal conditions, uniform nanorods with minor axes of 331.11 ± 85.90 nm were fabricated, and the rod fabrication yield reached nearly 100%. Paclitaxel, a classical chemotherapeutic drug, was successfully encapsulated into the obtained nanorods without altering their anisotropic morphology. Furthermore, the drug-loaded nanorods exhibited enhanced <i>in vitro</i> anti-tumor efficacy against A549 lung cancer cells. This methodology provides a facile route for preparing uniform nonspherical PLGA particles in the nanosize range, offering promising applications as drug carriers in nanomedicine. 2026-09-28T00:00:00Z Text Journal contribution 10.1021/acsomega.6c07438.s001 https://figshare.com/articles/journal_contribution/Preparation_of_Uniform_Nonspherical_PLGA_Particles_in_the_Nanosize_Range_by_Using_a_Two-Step_Premix_Membrane_Emulsification_Technique/34018704 CC BY-NC 4.0