2026-10-11T10:01:08Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34018701 2026-09-29T00:05:07Z category_12 category_13 category_14 category_39 category_734 category_61 category_64 category_128 category_133 category_135 portal_63 item_type_3 month_year_09_2026

Multi-Stage Transcriptomics Reveals TF- and lncRNA-Mediated Regulatory Networks Governing Glyphosate Resistance in Cyperus rotundus Shiqin Zhao (25133520) Lamei Wu (1867720) Tianbo Liu (1390855) Yanxuan Lu (25133523) Hui Zhao (7395) Hailin Cai (11405711) Min Liu (45756) Zhanghui Liu (19232464) Lianyang Bai (104850) Zi Luo (11880505) Lang Pan (5145221) Cell Biology Genetics Molecular Biology Ecology Biological Sciences not elsewhere classified Developmental Biology Cancer Hematology Plant Biology Computational Biology specific transcriptional reprogramming globally invasive weed site resistance process resistance regulatory network confidence candidate resistance suggesting multilayered regulation multiple long non stage rna sequencing iii developmental stage multilayered non glyphosate resistance strongest divergence integrating constitutive findings demonstrate developmentally coordinated coding rnas central position associated genes allelic copies abcc8 </ Glyphosate resistance in Cyperus rotundus, a globally invasive weed, is increasingly threatening crop production, yet its molecular mechanisms remain unclear. Here, we performed multi-stage RNA sequencing of tubers from glyphosate-resistant and -susceptible populations across three developmental stages to investigate the transcriptional dynamics underlying resistance. Differential expression analysis revealed extensive stage-specific transcriptional reprogramming, with the strongest divergence at the III developmental stage. Integrating constitutive and stage-dependent expression patterns identified 34 high-confidence candidate resistance-associated genes. Co-expression and regulatory network analyses uncovered coordinated transcriptional modules involving WRKY, NAC, ERF, MYB, bHLH, and ARF transcription factors together with multiple long non-coding RNAs. Notably, all allelic copies of <i>ABCC8</i> were consistently up-regulated in glyphosate-resistant plants, validated by RT-qPCR, and occupied a central position in the resistance regulatory network, suggesting multilayered regulation of detoxification. These findings demonstrate that glyphosate resistance in C. rotundus is a developmentally coordinated, multilayered non-target-site resistance process. 2026-09-28T00:00:00Z Dataset Dataset 10.1021/acs.jafc.6c09642.s002 https://figshare.com/articles/dataset/Multi-Stage_Transcriptomics_Reveals_TF-_and_lncRNA-Mediated_Regulatory_Networks_Governing_Glyphosate_Resistance_in_Cyperus_rotundus/34018701 CC BY-NC 4.0