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oai:figshare.com:article/34018580 2026-09-28T22:03:43Z category_1 category_4 category_21 category_272 category_873 category_39 category_931 category_133 category_134 portal_63 item_type_6 month_year_09_2026

Bimetallic Porphyrin-Based Metal–Organic Framework for Noninvasive Electrochemical Monitoring of Nitric Oxide in Plants and Fruits Yujiao Bai (14833743) Wenqing Gao (766108) Yujie Pan (8425296) Zhaoxuan Cheng (24150809) Aiyu Zhang (2352328) Lina Zhang (112887) Peihua Zhu (2081983) Jinghua Yu (279880) Biophysics Biochemistry Biotechnology Environmental Sciences not elsewhere classified Chemical Sciences not elsewhere classified Ecology Information Systems not elsewhere classified Plant Biology Virology wide linear range printed carbon electrodes platform enabled real gaseous signaling molecules climatic changes intensify 5 – 565 5 μm ), 14 μm ), cellular redox homeostasis fruit tissues subjected low detection limit 3 +</ sup porous mof structure noninvasive electrochemical monitoring fe – n 4 </ sub fruit tissues situ </ redox cycling noninvasive monitoring work highlights thermal treatment room temperature promising strategy nitric oxide ni acting mx ), mechanical wounding interference capability high sensitivity great importance frequently exposed facilitate rapid excessive accumulation excellent anti electronic advantages electron reservoir cooperative interaction bimetallic synergy bimetallic porphyrin active sites abiotic stresses As environmental and climatic changes intensify, plants and fruits are frequently exposed to abiotic stresses, leading to excessive accumulation of nitric oxide (NO) and disruption of cellular redox homeostasis, so noninvasive monitoring of NO is of great importance. In this study, MX@Fe/NiPor-MOF was constructed at room temperature by assembling a bimetallic porphyrin-based metal–organic framework (Fe/NiPor-MOF) with transition metal carbide nanosheets (MX), and further integrated with screen-printed carbon electrodes (SPCEs) for noninvasive NO detection in plants and fruits. The cooperative interaction between Fe/Ni bimetallic centers enabled efficient electron redistribution, with Ni acting as an electron reservoir to facilitate rapid and reversible Fe²⁺/Fe³⁺ redox cycling. Meanwhile, the porous MOF structure and highly conductive MX promoted the exposure of Fe–N₄/Ni–N₄ active sites and established efficient electron transport pathways. Benefiting from these structural and electronic advantages, the MX@Fe/NiPor-MOF/SPCE electrochemical sensor exhibited excellent electrocatalytic performance toward NO detection, including a wide linear range (0.5–565.5 μM), a low detection limit (0.14 μM), a high sensitivity (1.95 μA·μM^–1·cm^–2), and excellent anti-interference capability. As a proof of concept, the platform enabled real-time, noninvasive electrochemical monitoring of microscale NO release from plant and fruit tissues subjected to thermal treatment and mechanical wounding. This work highlights the role of bimetallic synergy in enhancing NO electrocatalysis and provides a promising strategy for <i>in situ</i> monitoring of gaseous signaling molecules in plant and fruit tissues. 2026-09-28T00:00:00Z Text Journal contribution 10.1021/acs.analchem.6c04451.s001 https://figshare.com/articles/journal_contribution/Bimetallic_Porphyrin-Based_Metal_Organic_Framework_for_Noninvasive_Electrochemical_Monitoring_of_Nitric_Oxide_in_Plants_and_Fruits/34018580 CC BY-NC 4.0