2026-10-08T06:54:41Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34018482 2026-09-28T21:06:15Z category_24778 item_type_12 month_year_09_2026

Experimental Protocol and Validation Proposal: In Vitro Evaluation of Dual Fuel Modulation (DFM) and Adaptive Resistance via the Epitranscriptomic DAMER–ALKBH5–ATF4 Axis in Glioblastoma Mustafa Al-wasabi (24553704) Cancer cell biology experimental protocol. In vitro validation <p dir="ltr">This protocol outlines an in vitro experimental strategy for validating Dual Fuel Modulation (DFM) in glioblastoma (GBM) models while simultaneously evaluating candidate adaptive resistance pathways. The study combines coordinated metabolic perturbation—glycolytic inhibition, System Xc⁻ blockade, polyunsaturated fatty acid enrichment, and partial mitochondrial modulation—with mechanistic assessment of ferroptosis. Six treatment groups are proposed to distinguish DFM efficacy, ferroptosis specificity, and resistance-axis contributions. The design prioritizes the epitranscriptomic DAMER–ALKBH5–ATF4 axis and BRD4-associated chromatin remodeling as candidate resistance mechanisms. Primary endpoints include cell viability, lipid peroxidation, NADPH/GSH status, ATF4/NRF2 activation, SLC7A11/GPX4 expression, m6A modification, and BRD4 occupancy. Statistical evaluation will use the Chou–Talalay combination index to assess synergy. This protocol provides a staged framework for evaluating whether resistance-axis inhibition restores DFM sensitivity and preserves a therapeutic window in non-malignant neural cells.</p> 2026-09-28T21:06:15Z Text Preprint 10.6084/m9.figshare.34018482.v1 https://figshare.com/articles/preprint/Experimental_Protocol_and_Validation_Proposal_In_Vitro_Evaluation_of_Dual_Fuel_Modulation_DFM_and_Adaptive_Resistance_via_the_Epitranscriptomic_DAMER_ALKBH5_ATF4_Axis_in_Glioblastoma/34018482 CC BY 4.0