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oai:figshare.com:article/34017926 2026-09-28T17:58:18Z category_1 category_4 category_915 category_12 category_14 category_16 category_21 category_46 category_734 category_61 category_69 category_133 portal_5 item_type_1 month_year_09_2026

<p>Z-disc Adhirons are specific for their targets.</p> Oliver Umney (20106066) Alistair P. Curd (25132880) Heather L. Martin (25132883) Tarek Lewis (25132886) Anna Ah-San Tang (25132889) Thembaninkosi Gaule (25132892) Iain W. Manfield (11978060) Harikrushnan Balasubramanian (25132895) Satya Khuon (263722) Jesse Aaron (197822) Michelle Peckham (3912823) Biophysics Biochemistry Physical Sciences not elsewhere classified Cell Biology Molecular Biology Physiology Biotechnology Immunology Biological Sciences not elsewhere classified Developmental Biology Inorganic Chemistry Plant Biology basic repeating unit div >< p similar repeating organisation determine protein organisation discs </ p cardiac muscle z 3 key proteins key role cardiac signalling 3 </ striated muscle used perpl underlying organisation relative positions pattern extraction located within joined together fluorescently labelled discs play analysis revealed analyse patterns 3d position <p>A and B show the results from Alphafold3 modelling for the Adhirons for Z1Z2 (A) and ZASP (B) with their target proteins. The structures are shown in Alphaphofold3 colours, to show the prediction accuracy and as ribbon structures to show potential sites of interaction. C. The specificity of the Z-disc Adhirons for their target proteins was confirmed by immunoprecipitations using the Adhiron His-Tag, His-Tag Dynabeads and GST-tagged target proteins, followed by immunoblotting for GST and His Tags. All Adhirons bound their targets with minimal cross-reactivity and no Adhirons bound to the End Z2 to Zr5 Titin protein. Ad – Adhiron, Cont. – control (an Adhiron with AAAA and AAE in the variable loops). Representative blots shown (S1 Raw Gel). N = 3. D. Example SPR data. Adhirons were immobilized on streptavidin-coated Series S sensor chips via C‑terminal biotin and differing concentrations of GST-ZASP PDZ, GST-Z1/Z2, GST‑α‑actinin‑2 CH domains and GST-end Z2 to Zr5 flowed over. Representative curves are shown. No binding was observed for GST- α‑actinin‑2 CH domains in this assay.</p> <p>(TIF)</p> 2026-09-28T18:02:26Z Image Figure 10.1371/journal.pcbi.1014309.s001 https://figshare.com/articles/figure/_p_Z-disc_Adhirons_are_specific_for_their_targets_p_/34017926 CC BY 4.0