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2026-09-28T17:54:31Z
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<p>SBF-SEM analysis of <i>atg18-mNG:loxP</i> parasite architecture.</p>
Yasmin Schmitz (11974910)
Moumita Sengupta (25132859)
Carola Schneider (3743791)
Tanja Ziesmann (3318936)
Franziska Hellmold (25132862)
Ute Distler (115774)
Rudolph Reimer (190562)
Joachim Michael Matz (25132865)
Biophysics
Biochemistry
Physical Sciences not elsewhere classified
Medicine
Microbiology
Cell Biology
Genetics
Infectious Diseases
related protein 18
div >< p
broad architectural destabilization
balance cargo influx
associated protein machinery
antimalarial drugs unchanged
3d electron microscopy
membrane sector phenocopied
endocytic membrane homeostasis
endocytic membrane dynamics
vacuole constantly fuses
specialized digestive vacuole
stage parasites hypersensitive
vacuolar membrane dynamics
efficient hemoglobin processing
degrading hemoglobin within
asexual parasite proliferation
plasmodium falciparum </
atpase assembly factor
atg18 links v
vacuolar membrane
vacuole ’
malaria parasites
atpase assembly
parasite ’
parasite survival
parasite death
incoming hemoglobin
pf </
vesicle trafficking
rendered ring
regulatory influence
quantitative live
proton pump
point mutation
multifaceted role
key regulator
interactions indicates
interact directly
findings establish
filled vesicles
direct interference
decrease sensitivity
<p><i>Pf</i>ATG18 loss was induced at the ring stage and parasites were analyzed at 36 hpi. <b>(</b><b>A)</b> Representative SBF-SEM section of a DMSO control parasite, highlighting subcellular features selected for segmentation. The apicoplast was readily distinguished from the mitochondrion by its stronger membrane contrast. <b>(B,C)</b> 3D reconstructions of parasite structures in DMSO- and RAP-treated cells, shown individually <b>(B)</b> or combined <b>(C)</b>. See also Supplemental Movies S1-S4 in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1014608#ppat.1014608.s013" target="_blank">S1 File</a>.</p> <p>(TIF)</p>
2026-09-28T18:08:16Z
Image
Figure
10.1371/journal.ppat.1014608.s003
https://figshare.com/articles/figure/_p_SBF-SEM_analysis_of_i_atg18-mNG_loxP_i_parasite_architecture_p_/34017803
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