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oai:figshare.com:article/34017761 2026-09-28T17:53:29Z category_1 category_4 category_915 category_7 category_12 category_13 category_14 category_16 category_873 category_734 category_61 category_128 category_135 portal_5 item_type_1 month_year_09_2026

Key enzymes of glycolysis and the TCA cycle exhibit nuclear activity. Ana Paula Menezes (25132835) Camila Gachet-Castro (11579152) Suzanne McGill (11916067) Richard Burchmore (178746) Ariel Silber (25132838) Maria Carolina Elias (9906069) Júlia P. C. da Cunha (25132841) Biophysics Biochemistry Physical Sciences not elsewhere classified Medicine Cell Biology Genetics Molecular Biology Physiology Chemical Sciences not elsewhere classified Biological Sciences not elsewhere classified Developmental Biology Hematology Computational Biology unique cellular features transition using hydroxyurea regulating fundamental processes pyruvate kinase activities maintaining genomic integrity hu ), followed cd ), represents specific expression patterns previously underappreciated role gene expression regulation canonical regulatory mechanisms >, potentially influencing trypanosoma cruzi </ purified nuclear fraction metabolic enzymes including gene expression metabolic enzymes cruzi </ pivotal role variant histones valuable model temporal compartmentalization specialized organelles revealing distinct results suggest provide insights proteins involved proteins identified protein synthesis nucleoside metabolisms nuclear region nuclear proteome nuclear division microtubule dynamics increased levels immunofluorescence supported identified 2 eukaryotic models etiological agent dna replication diverging eukaryotes citrate synthase chromosome condensation (A)Activity of hexokinase (HK), citrate synthase (CS), and pyruvate kinase (PK), glycerol kinase (GK) and pyrroline-5-carboxylate dehydrogenase (P5CDH) were obtained using either nuclear and cytoplasmic extracts. The controls used here were not found in chromatin and nuclear proteomes. Reactions without the enzyme substrates were used to rule out residual activity. Enzymatic activities were normalized with the total activities of parasite extract for each listed enzyme. Means ± SD of triplicate data (n = 3) are graphically represented. *p < 0.01; **p < 0.001; *** p < 0.0001. One-way ANOVA (Bonferroni’s Multiple Comparison Test). (B) KEGG map of the glycolysis and TCA cycle pathways. (C) Immunofluorescence analysis of hexokinase (HK), citrate synthase (CS), and glycerol kinase (GK) in T. cruzi epimastigotes expressing H2B.V-Myc as a nuclear marker. HK, CS, and GK were detected with Alexa Fluor 488-conjugated secondary antibody, H2B.V-Myc was detected with Alexa Fluor 555-conjugated secondary antibody, and DNA was stained with DAPI. Images were acquired using an Olympus BX51 fluorescence microscope controlled by Olympus cell^R software and processed/assembled using ImageJ. Bright-field, individual fluorescence channels, and merged images are shown. White arrows indicate the linear regions of interest drawn across the parasite body and nuclear region. 2026-09-28T17:59:59Z Image Figure 10.1371/journal.pntd.0013299.g005 https://figshare.com/articles/figure/_p_Key_enzymes_of_glycolysis_and_the_TCA_cycle_exhibit_nuclear_activity_p_/34017761 CC BY 4.0