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oai:figshare.com:article/34017758 2026-09-28T17:53:27Z category_1 category_4 category_915 category_7 category_12 category_13 category_14 category_16 category_873 category_734 category_61 category_128 category_135 portal_5 item_type_1 month_year_09_2026

<p>Analysis of nuclear metabolic enzymes across the <i>T. cruzi</i> cell cycle.</p> Ana Paula Menezes (25132835) Camila Gachet-Castro (11579152) Suzanne McGill (11916067) Richard Burchmore (178746) Ariel Silber (25132838) Maria Carolina Elias (9906069) Júlia P. C. da Cunha (25132841) Biophysics Biochemistry Physical Sciences not elsewhere classified Medicine Cell Biology Genetics Molecular Biology Physiology Chemical Sciences not elsewhere classified Biological Sciences not elsewhere classified Developmental Biology Hematology Computational Biology unique cellular features transition using hydroxyurea regulating fundamental processes pyruvate kinase activities maintaining genomic integrity hu ), followed cd ), represents specific expression patterns previously underappreciated role gene expression regulation canonical regulatory mechanisms >, potentially influencing trypanosoma cruzi </ purified nuclear fraction metabolic enzymes including gene expression metabolic enzymes cruzi </ pivotal role variant histones valuable model temporal compartmentalization specialized organelles revealing distinct results suggest provide insights proteins involved proteins identified protein synthesis nucleoside metabolisms nuclear region nuclear proteome nuclear division microtubule dynamics increased levels immunofluorescence supported identified 2 eukaryotic models etiological agent dna replication diverging eukaryotes citrate synthase chromosome condensation <p>Proportion of nuclear proteins associated with metabolism (5.96%) identified using GO terms related to key metabolic processes, including “cellular amino acid metabolic process” (GO:0006520), “carbohydrate metabolic process” (GO:0005975), “pyruvate metabolic process” (GO:0006090), “ATP metabolic process” (GO:00046034), “nucleotide metabolic process” (GO:0009116), “nucleoside metabolic process” (GO:0009117), “lipid metabolic process” (GO:0006629), and “generation of precursor metabolites and energy” (GO:0006091). <b>(B)</b> KEGG pathway enrichment analysis of the metabolic enzymes identified in <b>(A)</b>, performed using TriTrypDB’s tool with a Bonferroni-corrected p-value cutoff of 0.05. Enriched pathways are shown according to fold enrichment. <b>(C)</b> Venn diagram comparing the nuclear proteins identified in this study (CC_PN) with those present in the nuclear proteome (CC_PT) (De Castro Moreira Dos Santos et al., 2015) and those at chromatin proteome (Chr_P) (De Jesus et al., 2017). <b>(D)</b> Hierarchical clustering analysis of all protein expression profiles across different cell cycle phases. The protein data abundance was normalized before clustering by the Euclidean distance ratio. <b>(E)</b> Venn diagram between the nuclear cell cycle proteins identified in this study (CC_NP) and the Cell cycle proteome (CC_P) (Santos Júnior et al., 2021). <b>(F)</b> Box plots representing the relative abundance (%) of nuclear metabolic proteins found in (A) and ribosomal proteins across cell cycle phases.</p> 2026-09-28T17:59:59Z Image Figure 10.1371/journal.pntd.0013299.g004 https://figshare.com/articles/figure/_p_Analysis_of_nuclear_metabolic_enzymes_across_the_i_T_cruzi_i_cell_cycle_p_/34017758 CC BY 4.0