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oai:figshare.com:article/34017749 2026-09-28T17:53:23Z category_1 category_4 category_915 category_7 category_12 category_13 category_14 category_16 category_873 category_734 category_61 category_128 category_135 portal_5 item_type_1 month_year_09_2026

Cell cycle-associated variation of histones, kinases, and nucleotide metabolism-related proteins in the T. cruzi nuclear proteome. Ana Paula Menezes (25132835) Camila Gachet-Castro (11579152) Suzanne McGill (11916067) Richard Burchmore (178746) Ariel Silber (25132838) Maria Carolina Elias (9906069) Júlia P. C. da Cunha (25132841) Biophysics Biochemistry Physical Sciences not elsewhere classified Medicine Cell Biology Genetics Molecular Biology Physiology Chemical Sciences not elsewhere classified Biological Sciences not elsewhere classified Developmental Biology Hematology Computational Biology unique cellular features transition using hydroxyurea regulating fundamental processes pyruvate kinase activities maintaining genomic integrity hu ), followed cd ), represents specific expression patterns previously underappreciated role gene expression regulation canonical regulatory mechanisms >, potentially influencing trypanosoma cruzi </ purified nuclear fraction metabolic enzymes including gene expression metabolic enzymes cruzi </ pivotal role variant histones valuable model temporal compartmentalization specialized organelles revealing distinct results suggest provide insights proteins involved proteins identified protein synthesis nucleoside metabolisms nuclear region nuclear proteome nuclear division microtubule dynamics increased levels immunofluorescence supported identified 2 eukaryotic models etiological agent dna replication diverging eukaryotes citrate synthase chromosome condensation (A) Relative abundance of canonical histones during G1/S, S, and G2/M phases. (B) Relative abundance of histone variants during G1/S, S, and G2/M phases. (C) Relative abundance profiles of selected kinases detected in the nuclear proteome, including metabolic kinases and signaling-associated kinases. (D) Relative abundance of selected proteins associated with nucleotide metabolism across the cell cycle. Statistical significance was assessed by ANOVA (*p < 0.05, **p < 0.01). (E) Schematic representation of nucleotide metabolism in T. cruzi, including pyrimidine de novo and salvage pathways and the purine salvage pathway. Enzymes are color-coded according to their detection and abundance variation across the cell cycle: orange, identified but not quantified; green, no significant variation; red, increased abundance in G1/S compared with S and G2/M; gray, decreased abundance in G2/M compared with S and G1/S; and white, not detected. Pyrimidine metabolism: carbamoyl-phosphate synthetase II (CPSII, EC:6.3.5.5), aspartate carbamoyltransferase (ATCase, EC:2.1.3.2), dihydroorotase (DHOase, EC:3.5.2.3), dihydroorotate dehydrogenase (DHODH, EC:1.3.98.1), orotate phosphoribosyltransferase (OPRT, EC:2.4.2.10), orotidine-5-phosphate decarboxylase (OMP, EC:4.1.1.23), uracil phosphoribosyltransferase (UPT, EC:2.4.2.9), UMP-CMP kinase 2 (UMPK, EC:2.7.4.22), cytidine triphosphate synthase (CTPS, EC:6.3.4.2), uridine-cytidine kinase (UCK, EC:2.7.1.48), nucleoside diphosphate kinase (NDK, EC:2.7.4.6), ribonucleoside-diphosphate reductase (RNR, EC:1.17.4.1), 5′-deoxynucleotidase (5′DN, EC:3.1.3.89), thymidylate kinase (TK, EC:2.7.4.9), and phosphoribosylpyrophosphate synthetase (PRPS, EC:2.7.6.1). Purine metabolism: inosine-adenosine-guanosine-nucleoside hydrolase (IGNH, EC:3.2.2.1), hypoxanthine-guanine phosphoribosyltransferase (HGPRT, EC:2.4.2.8), inosine-5′-monophosphate dehydrogenase (IMPDH/INMD, EC:1.1.1.205), guanylate kinase (GK, EC:2.7.4.8), adenosine kinase (AK, EC:2.7.1.20), and adenylate kinase (ADK, EC:2.7.4.3). Abbreviations: PRPP, phosphoribosyl pyrophosphate; AMP, adenosine monophosphate; ADP, adenosine diphosphate; ATP, adenosine triphosphate; dADP, deoxyadenosine diphosphate; dATP, deoxyadenosine triphosphate; IMP, inosine monophosphate; GMP, guanosine monophosphate; GDP, guanosine diphosphate; GTP, guanosine triphosphate; dGDP, deoxyguanosine diphosphate; dGTP, deoxyguanosine triphosphate; XMP, xanthosine monophosphate; UMP, uridine monophosphate; UDP, uridine diphosphate; UTP, uridine triphosphate; CMP, cytidine monophosphate; CDP, cytidine diphosphate; CTP, cytidine triphosphate; dCDP, deoxycytidine diphosphate; dCTP, deoxycytidine triphosphate; dTDP, deoxythymidine diphosphate; dTTP, deoxythymidine triphosphate. 2026-09-28T17:59:59Z Image Figure 10.1371/journal.pntd.0013299.g002 https://figshare.com/articles/figure/_p_Cell_cycle-associated_variation_of_histones_kinases_and_nucleotide_metabolism-related_proteins_in_the_T_cruzi_nuclear_proteome_p_/34017749 CC BY 4.0