2026-10-10T09:51:11Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34017746 2026-09-28T17:53:22Z category_1 category_4 category_915 category_7 category_12 category_13 category_14 category_16 category_873 category_734 category_61 category_128 category_135 portal_5 item_type_1 month_year_09_2026

<p>Characterization of Nuclear Protein Extracts and Histone Abundance During the <i>T. cruzi</i> Cell Cycle.</p> Ana Paula Menezes (25132835) Camila Gachet-Castro (11579152) Suzanne McGill (11916067) Richard Burchmore (178746) Ariel Silber (25132838) Maria Carolina Elias (9906069) Júlia P. C. da Cunha (25132841) Biophysics Biochemistry Physical Sciences not elsewhere classified Medicine Cell Biology Genetics Molecular Biology Physiology Chemical Sciences not elsewhere classified Biological Sciences not elsewhere classified Developmental Biology Hematology Computational Biology unique cellular features transition using hydroxyurea regulating fundamental processes pyruvate kinase activities maintaining genomic integrity hu ), followed cd ), represents specific expression patterns previously underappreciated role gene expression regulation canonical regulatory mechanisms >, potentially influencing trypanosoma cruzi </ purified nuclear fraction metabolic enzymes including gene expression metabolic enzymes cruzi </ pivotal role variant histones valuable model temporal compartmentalization specialized organelles revealing distinct results suggest provide insights proteins involved proteins identified protein synthesis nucleoside metabolisms nuclear region nuclear proteome nuclear division microtubule dynamics increased levels immunofluorescence supported identified 2 eukaryotic models etiological agent dna replication diverging eukaryotes citrate synthase chromosome condensation <p><b>(A)</b> Representative flow cytometry histograms indicate the distribution of the DNA content in the experimental groups G1/S, S, G2/M and asynchronous control. <b>(B)</b> Immunofluorescence images of epimastigotes (Epi, top row) and isolated nuclear fractions (Nuclei, bottom row). DNA was stained with DAPI (blue), and the flagellum was labeled with the anti-flagellum monoclonal antibody mAb 2F6 (green). Brightfield images are shown on the right. N, nucleus; K, kinetoplast. <b>(C)</b> SDS-PAGE (15%) of epimastigote and nuclear extracts stained with Coomassie blue, showing enrichment of histones (~15 kDa) in the nuclear fraction. <b>(D)</b> Representative western blot assay showing the presence of nuclear marker protein (anti-H3) and the absence of ant-HSP70 (endoplasmatic reticulum), anti-aldolase (glycosomal) in the nuclear fraction and whole cell lysate. <b>(E)</b> Venn diagram showing the overlap of proteins identified in the nuclear proteome across the three cell cycle phases analyzed. <b>(F)</b> Venn diagram comparing proteins identified in our nuclear cell cycle proteome (CC_PN, green) with those previously reported in the <i>T. cruzi</i> nuclear proteome (PN, orange) (1) and chromatin-associated proteome (Chr_P, blue) (2).</p> 2026-09-28T17:59:59Z Image Figure 10.1371/journal.pntd.0013299.g001 https://figshare.com/articles/figure/_p_Characterization_of_Nuclear_Protein_Extracts_and_Histone_Abundance_During_the_i_T_cruzi_i_Cell_Cycle_p_/34017746 CC BY 4.0