2026-10-12T02:34:00Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34017713 2026-09-28T17:53:07Z category_1 category_4 category_915 category_7 category_12 category_13 category_14 category_16 category_873 category_734 category_61 category_128 category_135 portal_5 item_type_1 month_year_09_2026

<p>Representative deconvolved Z-stack optical sections of <i>T. cruzi</i> epimastigotes stained for (A) hexokinase (HK), (B) citrate synthase (CS), and (C) glycerol kinase (GK), together with H2B.</p> Ana Paula Menezes (25132835) Camila Gachet-Castro (11579152) Suzanne McGill (11916067) Richard Burchmore (178746) Ariel Silber (25132838) Maria Carolina Elias (9906069) Júlia P. C. da Cunha (25132841) Biophysics Biochemistry Physical Sciences not elsewhere classified Medicine Cell Biology Genetics Molecular Biology Physiology Chemical Sciences not elsewhere classified Biological Sciences not elsewhere classified Developmental Biology Hematology Computational Biology unique cellular features transition using hydroxyurea regulating fundamental processes pyruvate kinase activities maintaining genomic integrity hu ), followed cd ), represents specific expression patterns previously underappreciated role gene expression regulation canonical regulatory mechanisms >, potentially influencing trypanosoma cruzi </ purified nuclear fraction metabolic enzymes including gene expression metabolic enzymes cruzi </ pivotal role variant histones valuable model temporal compartmentalization specialized organelles revealing distinct results suggest provide insights proteins involved proteins identified protein synthesis nucleoside metabolisms nuclear region nuclear proteome nuclear division microtubule dynamics increased levels immunofluorescence supported identified 2 eukaryotic models etiological agent dna replication diverging eukaryotes citrate synthase chromosome condensation <p>V-Myc and DAPI. HK, CS, and GK were detected with Alexa Fluor 488-conjugated secondary antibody, H2B.V-Myc was detected with Alexa Fluor 555-conjugated secondary antibody, and DNA was stained with DAPI. Images were acquired using an Olympus BX51 fluorescence microscope equipped with a 100 × oil-immersion objective and controlled by Olympus cell^R software. Z-stacks were acquired as sequential optical sections through the parasite body and deconvolved before visualization. Bright-field, Alexa Fluor 488, Alexa Fluor 555, DAPI, and merged channels are shown. Scale bars, 100 µm.</p> <p>(TIF)</p> 2026-09-28T17:59:59Z Image Figure 10.1371/journal.pntd.0013299.s007 https://figshare.com/articles/figure/_p_Representative_deconvolved_Z-stack_optical_sections_of_i_T_cruzi_i_epimastigotes_stained_for_A_hexokinase_HK_B_citrate_synthase_CS_and_C_glycerol_kinase_GK_together_with_H2B_p_/34017713 CC BY 4.0