2026-10-14T07:23:39Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34017710 2026-09-28T17:53:05Z category_1 category_4 category_915 category_7 category_12 category_13 category_14 category_16 category_873 category_734 category_61 category_128 category_135 portal_5 item_type_1 month_year_09_2026

<p>Fluorescence intensity line-profile analysis of metabolic enzyme staining relative to nuclear markers in <i>T. cruzi.</i></p> Ana Paula Menezes (25132835) Camila Gachet-Castro (11579152) Suzanne McGill (11916067) Richard Burchmore (178746) Ariel Silber (25132838) Maria Carolina Elias (9906069) Júlia P. C. da Cunha (25132841) Biophysics Biochemistry Physical Sciences not elsewhere classified Medicine Cell Biology Genetics Molecular Biology Physiology Chemical Sciences not elsewhere classified Biological Sciences not elsewhere classified Developmental Biology Hematology Computational Biology unique cellular features transition using hydroxyurea regulating fundamental processes pyruvate kinase activities maintaining genomic integrity hu ), followed cd ), represents specific expression patterns previously underappreciated role gene expression regulation canonical regulatory mechanisms >, potentially influencing trypanosoma cruzi </ purified nuclear fraction metabolic enzymes including gene expression metabolic enzymes cruzi </ pivotal role variant histones valuable model temporal compartmentalization specialized organelles revealing distinct results suggest provide insights proteins involved proteins identified protein synthesis nucleoside metabolisms nuclear region nuclear proteome nuclear division microtubule dynamics increased levels immunofluorescence supported identified 2 eukaryotic models etiological agent dna replication diverging eukaryotes citrate synthase chromosome condensation <p>Representative bright-field and merged immunofluorescence images of <i>T. cruzi</i> epimastigotes stained for hexokinase (HK), citrate synthase (CS), or glycerol kinase (GK), together with H2B.V-Myc and DAPI. HK, CS, and GK were detected with Alexa Fluor 488-conjugated secondary antibody, H2B.V-Myc was detected with Alexa Fluor 555-conjugated secondary antibody, and DNA was stained with DAPI. Images were acquired using an Olympus BX51 fluorescence microscope controlled by Olympus cell^R software. White arrows indicate the linear regions of interest drawn across the parasite body and nuclear region. The same linear ROI was applied to the Alexa Fluor 488, Alexa Fluor 555, and DAPI channels, and fluorescence intensity values were extracted along the ROI using Olympus cell^R. Intensity values were normalized and plotted as line profiles in GraphPad Prism to compare the spatial distribution of the three fluorescence signals.</p> <p>(TIF)</p> 2026-09-28T17:59:59Z Image Figure 10.1371/journal.pntd.0013299.s006 https://figshare.com/articles/figure/_p_Fluorescence_intensity_line-profile_analysis_of_metabolic_enzyme_staining_relative_to_nuclear_markers_in_i_T_cruzi_i_p_/34017710 CC BY 4.0