2026-10-10T03:29:50Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34017698 2026-09-28T17:53:00Z category_1 category_4 category_915 category_7 category_12 category_13 category_14 category_16 category_873 category_734 category_61 category_128 category_135 portal_5 item_type_1 month_year_09_2026

<p>Nuclear proteome dynamics: PCA clustering, protein abundance profiling, and GO-based nuclear enrichment analysis.</p> Ana Paula Menezes (25132835) Camila Gachet-Castro (11579152) Suzanne McGill (11916067) Richard Burchmore (178746) Ariel Silber (25132838) Maria Carolina Elias (9906069) Júlia P. C. da Cunha (25132841) Biophysics Biochemistry Physical Sciences not elsewhere classified Medicine Cell Biology Genetics Molecular Biology Physiology Chemical Sciences not elsewhere classified Biological Sciences not elsewhere classified Developmental Biology Hematology Computational Biology unique cellular features transition using hydroxyurea regulating fundamental processes pyruvate kinase activities maintaining genomic integrity hu ), followed cd ), represents specific expression patterns previously underappreciated role gene expression regulation canonical regulatory mechanisms >, potentially influencing trypanosoma cruzi </ purified nuclear fraction metabolic enzymes including gene expression metabolic enzymes cruzi </ pivotal role variant histones valuable model temporal compartmentalization specialized organelles revealing distinct results suggest provide insights proteins involved proteins identified protein synthesis nucleoside metabolisms nuclear region nuclear proteome nuclear division microtubule dynamics increased levels immunofluorescence supported identified 2 eukaryotic models etiological agent dna replication diverging eukaryotes citrate synthase chromosome condensation <p>(A) Principal-component analysis of quadruplicate samples in G1/S (orange), S (red) and G2/M (green). (B) Representation of the normalized relative protein abundance profile obtained through TMT analysis. Each bar corresponds to a replicate and reflects its abundance in the experimental samples. Error bars represent standard deviations from quadruplicate measurements. (C) Enrichment of nuclear proteins by Gene Ontology (GO) analysis. Word cloud demonstrates the enrichment of terms in the cellular components category (GO) for groups G1/S, S, and G2/M, respectively. The nuclei enrichment was evaluated using the TritrypDb, and the significance cutoff for the p-value <0.05. Font sizes in the word cloud are proportional to -log10 of the adjusted p-value for each enriched GO term.</p> <p>(TIF)</p> 2026-09-28T17:59:59Z Image Figure 10.1371/journal.pntd.0013299.s002 https://figshare.com/articles/figure/_p_Nuclear_proteome_dynamics_PCA_clustering_protein_abundance_profiling_and_GO-based_nuclear_enrichment_analysis_p_/34017698 CC BY 4.0