2026-10-11T12:44:59Z
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2026-09-28T17:52:54Z
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<p>Transcriptomic divergence of medulla neuropil glia along development.</p>
Amanda A. G. Ferreira (25132828)
Sergio Córdoba (645937)
Raghuvanshi Rajesh (25132831)
Ben Jiwon Choi (25132834)
Claude Desplan (45436)
Cell Biology
Genetics
Neuroscience
Environmental Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Developmental Biology
Cancer
Hematology
Plant Biology
innovative computational approach
genetic markers expressed
div >< p
subcellular mrna compartmentalization
optic lobe glia
two large single
detailed transcriptomic analysis
identify mrna species
glial diversity observed
adult visual system
glial cell bodies
glial cell types
understand glial development
visual system
cell mrna
cell bodies
glial types
glial cells
glia types
adult stages
cell class
cell atlas
neuropil glia
chiasm glia
work provides
transcriptionally appear
pupal stages
helps explain
gradually change
drosophila </
distinct mrnas
differentially localized
developmental trajectories
developmental single
comprehensively annotated
also present
allowed us
<p><b>(A)</b> UMAP of medulla neuropil glia (mng) cells showing the divergence of astrocyte-like (ALG) and ensheathing (EG) glial cell types. The terminal fates identified in the adult dataset are circled. <b>(B)</b> UMAP showing cells colored by their developmental stages of origin. <b>(C)</b> UMAP-based subsetting of cells at the fork (colored red) between ALG and EG adult fates. Inset displays the proportion of red cells that belong to each developmental stage. Note that the most represented stage at the trajectory fork is P30. <b>(D)</b> UMAP showing pseudotime alignment of mng glial cells. This pseudotime embedding is used for analyzing gene expression dynamics in (E). <b>(E)</b> Heatmap of dynamically regulated TFs along pseudotime highlighting putative TFs associated with mng divergence (dashed box) and canonical Notch effector genes (blue arrowheads). <b>(F, F’)</b> L3 brain showing the lamina (F) and medulla (F’) neuropil glia stained for Hey (magenta) and Repo (cyan) antibodies. Both lamina and medulla neuropil glia express Hey after specification (full arrowheads) and turn it off shortly after (empty arrowheads). eg = epithelial glia, mg = marginal glia and mng = medulla neuropil glia. The code used to generate the graphs can be found at <a href="https://zenodo.org/records/21890456" target="_blank">https://zenodo.org/records/21890456</a>.</p>
2026-09-28T17:57:37Z
Image
Figure
10.1371/journal.pbio.3004015.g005
https://figshare.com/articles/figure/_p_Transcriptomic_divergence_of_medulla_neuropil_glia_along_development_p_/34017688
CC BY 4.0