2026-10-11T17:22:06Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34017685 2026-09-28T17:52:52Z category_12 category_13 category_15 category_272 category_734 category_61 category_64 category_128 category_133 portal_5 item_type_1 month_year_09_2026

<p>Developmental diversification of neuropil glia.</p> Amanda A. G. Ferreira (25132828) Sergio Córdoba (645937) Raghuvanshi Rajesh (25132831) Ben Jiwon Choi (25132834) Claude Desplan (45436) Cell Biology Genetics Neuroscience Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Developmental Biology Cancer Hematology Plant Biology innovative computational approach genetic markers expressed div >< p subcellular mrna compartmentalization optic lobe glia two large single detailed transcriptomic analysis identify mrna species glial diversity observed adult visual system glial cell bodies glial cell types understand glial development visual system cell mrna cell bodies glial types glial cells glia types adult stages cell class cell atlas neuropil glia chiasm glia work provides transcriptionally appear pupal stages helps explain gradually change drosophila </ distinct mrnas differentially localized developmental trajectories developmental single comprehensively annotated also present allowed us <p><b>(A)</b> Left, UMAPs representing all glial clusters from L3, P15, P30, P50, P70 and adult stage optic lobes. Right, UMAP integration of all developmental stages; cells are color-coded according to their stage of origin. Blue arrows highlight glial cells that show a gradual developmental trajectory from L3 to adult in our integration. Red arrows indicate trajectories where glial cells that are initially similar bifurcate into different terminal fates. Circled in black are glial cells that do not show a developmental trajectory from L3 to adult according to the integration. Dashed lines represent trajectories that do not contain cells from larval stage. <b>(B)</b> UMAP showing isolated medulla neuropil glia from the integrated dataset in (A), where the divergent trajectories of medulla astrocyte-like (ALG) and can be observed. <i>Hand</i> displays a salt-and-pepper expression that is restricted at the beginning of the trajectory (L3). <b>(C–E)</b> L3 expression of <i>10C12</i>-Gal4, <i>54H11</i>-Gal5, and <i>Hand-T2A</i>-Gal4 lines, driving the UAS-nlsGFP-PEST (C and E) or UAS-LifeActGFP (D) in cyan. Ncad (yellow) is used to visualize the neuropil and Repo (magenta) to label all glia. White dashed boxes delineate the area magnified in the panels below. <i>10C12</i>-Gal4 is expressed specifically in epithelial and marginal glia, as well as in Lamina wide field neurons. <i>54H11</i>-Gal4 labels epithelial and marginal glia and medulla neuropil glia. <i>Hand-T2A</i>-Gal4 expression is only observed in some medulla neuropil glia (filled arrowheads), and absent in others (empty arrowheads). <b>(F–H)</b> FLEXAMP memory cassette is used to immortalize L3 expression of the Gal4 lines in C–E and to observe the morphology of adult glia. Only UAS-myrGFP expression is shown, and the neuropil shape is delineated in yellow dashed lines. Lamina and close-ups of medulla neuropil are indicated. Note that epithelial and marginal glia, that are indistinguishable at L3, diverge into specific morphologies and localizations in the adult (F and G). Tracings of L3 medulla neuropil glia show that they give rise to all morphotypes (ensheathing and astrocyte-like) and localizations (proximal, lateral and distal) of adult medulla neuropil glia (G and H). xgo = outer chiasm glia, mng = medulla neuropil glia, lng = lamina neuropil glia, eg = epithelial glia, mg = marginal glia. Gal4-UAS (<i>>></i>). Scale bar = 20 µm. The code used to generate the graphs can be found at <a href="https://zenodo.org/records/21890456" target="_blank">https://zenodo.org/records/21890456</a>.</p> 2026-09-28T17:57:37Z Image Figure 10.1371/journal.pbio.3004015.g004 https://figshare.com/articles/figure/_p_Developmental_diversification_of_neuropil_glia_p_/34017685 CC BY 4.0