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oai:figshare.com:article/34017673 2026-09-28T17:52:48Z category_12 category_13 category_15 category_272 category_734 category_61 category_64 category_128 category_133 portal_5 item_type_1 month_year_09_2026

Identification of new glia-specific marker genes. Amanda A. G. Ferreira (25132828) Sergio Córdoba (645937) Raghuvanshi Rajesh (25132831) Ben Jiwon Choi (25132834) Claude Desplan (45436) Cell Biology Genetics Neuroscience Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Developmental Biology Cancer Hematology Plant Biology innovative computational approach genetic markers expressed div >< p subcellular mrna compartmentalization optic lobe glia two large single detailed transcriptomic analysis identify mrna species glial diversity observed adult visual system glial cell bodies glial cell types understand glial development visual system cell mrna cell bodies glial types glial cells glia types adult stages cell class cell atlas neuropil glia chiasm glia work provides transcriptionally appear pupal stages helps explain gradually change drosophila </ distinct mrnas differentially localized developmental trajectories developmental single comprehensively annotated also present allowed us (A) Schematic representing how glial cell clusters were defined. Pearson correlation between the transcriptome of the clusters with that of bulk transcriptome of repo-positive and elav-positive cells was used to define glial clusters. The defined clusters were used to find new glial markers. (B) Dot plot showing the newly identified glial markers in the larva (L3). Numbers identify individual glial clusters, and “Non-Glial Cells” represent the combined gene expression of all the remaining cells from the whole larva dataset from Konstantinides and colleagues, 2022. The color of the dots indicates the mean normalized gene expression across all cells in a group (Average Expression), while the dot size indicates the fraction of cells with nonzero expression (Percent Expressed). (C) Schematic representation of the L3 optic lobe, indicating the position of the different glial cell types (in pink) and the localization of the neuropils (dark gray). The blue section represents the plane imaged in the immunostaining shown in (F-F’’’). (D) Dot plot showing the newly identified glial markers in the adult dataset per glial cluster. “Non-Glial Cells” represent the combined gene expression of all the remaining cells from the whole adult stage dataset from Özel and colleagues, 2021. (E) Schematic representation of the adult optic lobe, indicating the position of the different glial cell types (in pink) and the localization of the neuropils (dark gray). (F-F’’’) Immunostaining of the larval (L3) optic lobe showing the localization of Repo, CG32032 and AnxB9 antibodies. (G-G’’’) Immunostaining of the adult optic lobe showing the localization of Repo, CG32032 and AnxB9 antibodies. CB, Central Brain, fg = fenestrated glia, pg = perineurial glia, spg = subperineurial glia, dsg = distal satellite glia, psg = proximal satellite glia, eg = epithelial glia, mg = marginal glia, xgo = outer chiasm glia, xgi = inner chiasm glia, cxg = cortex glia, mng = medulla neuropil glia, rwg = retina wrapping glia. Scale bar = 20 µm. The code used to generate the graphs can be found in <a href="https://zenodo.org/records/21890456" target="_blank">https://zenodo.org/records/21890456</a>. 2026-09-28T17:57:37Z Image Figure 10.1371/journal.pbio.3004015.g001 https://figshare.com/articles/figure/_p_Identification_of_new_glia-specific_marker_genes_p_/34017673 CC BY 4.0