2026-10-14T05:33:48Z
https://api.figshare.com/v2/oai
oai:figshare.com:article/34017637
2026-09-28T17:52:36Z
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<p>Validation of AnxB9 and CG32032 antibodies.</p>
Amanda A. G. Ferreira (25132828)
Sergio Córdoba (645937)
Raghuvanshi Rajesh (25132831)
Ben Jiwon Choi (25132834)
Claude Desplan (45436)
Cell Biology
Genetics
Neuroscience
Environmental Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Developmental Biology
Cancer
Hematology
Plant Biology
innovative computational approach
genetic markers expressed
div >< p
subcellular mrna compartmentalization
optic lobe glia
two large single
detailed transcriptomic analysis
identify mrna species
glial diversity observed
adult visual system
glial cell bodies
glial cell types
understand glial development
visual system
cell mrna
cell bodies
glial types
glial cells
glia types
adult stages
cell class
cell atlas
neuropil glia
chiasm glia
work provides
transcriptionally appear
pupal stages
helps explain
gradually change
drosophila </
distinct mrnas
differentially localized
developmental trajectories
developmental single
comprehensively annotated
also present
allowed us
<p><b>(A)</b><i>wg/xg</i><sup><i>O</i></sup>-Gal4 driver is used to express UAS-nlsGFP-PEST (cyan) in wrapping and outer chiasma glia (cyan dashed contour in lower panels), which show higher levels of AnxB9 antibody (magenta and separate channel below). Repo (gray) is used to identify glial cells. Control UAS-mCherry-RNAi (A), and two different interference RNA constructs against <i>AnxB9</i> <b>(A’, A’’)</b> are expressed, showing a cell type-specific reduction of AnxB9 signal in outer chiasma glia. <b>(B)</b> <i>10C12</i>-Gal4 driver is used to express UAS-nlsGFP-PEST (cyan) in lamina neuropil glia, which display higher levels of CG32032 antibody. We did not observe any difference in CG32032 signal in optic lobes expressing UAS-mCherry-RNAi controls (B) or a UAS-CG32032-RNAi construct <b>(B’)</b> in lamina neuropil glia. Scale bar = 20 µm.</p> <p>(TIFF)</p>
2026-09-28T17:57:37Z
Image
Figure
10.1371/journal.pbio.3004015.s007
https://figshare.com/articles/figure/_p_Validation_of_AnxB9_and_CG32032_antibodies_p_/34017637
CC BY 4.0