2026-10-14T17:14:37Z
https://api.figshare.com/v2/oai
oai:figshare.com:article/34017631
2026-09-28T17:52:34Z
category_12
category_13
category_15
category_272
category_734
category_61
category_64
category_128
category_133
portal_5
item_type_1
month_year_09_2026
<p>scRNA-seq reveals subcellular distribution of mRNAs.</p>
Amanda A. G. Ferreira (25132828)
Sergio Córdoba (645937)
Raghuvanshi Rajesh (25132831)
Ben Jiwon Choi (25132834)
Claude Desplan (45436)
Cell Biology
Genetics
Neuroscience
Environmental Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Developmental Biology
Cancer
Hematology
Plant Biology
innovative computational approach
genetic markers expressed
div >< p
subcellular mrna compartmentalization
optic lobe glia
two large single
detailed transcriptomic analysis
identify mrna species
glial diversity observed
adult visual system
glial cell bodies
glial cell types
understand glial development
visual system
cell mrna
cell bodies
glial types
glial cells
glia types
adult stages
cell class
cell atlas
neuropil glia
chiasm glia
work provides
transcriptionally appear
pupal stages
helps explain
gradually change
drosophila </
distinct mrnas
differentially localized
developmental trajectories
developmental single
comprehensively annotated
also present
allowed us
<p><b>(A)</b> UMAP representing the L3 glial clusters annotated accordingly to cluster numbers prior to annotation (<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3004015#pbio.3004015.g002" target="_blank">Fig 2B</a> and <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3004015#pbio.3004015.s009" target="_blank">S1 Table</a>). Chiasm glia clusters are highlighted in blue and cortex glia clusters in pink. PC = Cellular Processes, CB = Cell Body. <b>(B)</b> Heatmap representing the top markers for each cluster (high expression in yellow, and low expression in purple). The chiasm glia cluster 12 and 4 are highly similar, sharing the same markers, as is the case for the cortex glia clusters 20, 7, 1, and 3. <b>(C, D)</b> Dissociated cells from L3 optic lobe expressing membrane-bound GFP in chiasm glia. Hoechst labels cellular nuclei. Chiasm glia with nucleus (C) and Chiasm glia fragments without nucleus (D). Scale bar = 20 µm. The code used to generate the graphs can be found at <a href="https://zenodo.org/records/21890456" target="_blank">https://zenodo.org/records/21890456</a>.</p> <p>(TIFF)</p>
2026-09-28T17:57:37Z
Image
Figure
10.1371/journal.pbio.3004015.s005
https://figshare.com/articles/figure/_p_scRNA-seq_reveals_subcellular_distribution_of_mRNAs_p_/34017631
CC BY 4.0