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oai:figshare.com:article/34017619 2026-09-28T17:52:30Z category_12 category_13 category_15 category_272 category_734 category_61 category_64 category_128 category_133 portal_5 item_type_1 month_year_09_2026

Cluster identity in the integrated dataset and the origin of lobula neuropil glia. Amanda A. G. Ferreira (25132828) Sergio Córdoba (645937) Raghuvanshi Rajesh (25132831) Ben Jiwon Choi (25132834) Claude Desplan (45436) Cell Biology Genetics Neuroscience Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Developmental Biology Cancer Hematology Plant Biology innovative computational approach genetic markers expressed div >< p subcellular mrna compartmentalization optic lobe glia two large single detailed transcriptomic analysis identify mrna species glial diversity observed adult visual system glial cell bodies glial cell types understand glial development visual system cell mrna cell bodies glial types glial cells glia types adult stages cell class cell atlas neuropil glia chiasm glia work provides transcriptionally appear pupal stages helps explain gradually change drosophila </ distinct mrnas differentially localized developmental trajectories developmental single comprehensively annotated also present allowed us (A, B) UMAP representing the integration of all glial cells from L3, P15, P30, P50, P70, and adult stages (see <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3004015#pbio.3004015.g004" target="_blank">Fig 4A</a>). Only larval cells are colored in (A), and adult cells in (B). Note that pseudocartridge, fenestrated, and chalice glia are not present in the L3 dataset (dashed circles) and the expansion of neuropil glia cell types. (C) Hand-T2A-Gal4 drives the expression of a memory-lacZ cassette. β-Gal (cyan and separate channel below) positive cells all express Repo (magenta), and are located in the proximal, lateral, and distal surfaces of the medulla neuropil, labeled by anti-Brp antibody (yellow and yellow dashed line below). (D) Section of a third instar larva optic lobe showing the lobula complex neuropil (Ncad, yellow and dashed contour). The border between the central brain (CB) and the optic lobe (OL) is shown by a dashed line. The expression of UAS-LifeActGFP (cyan, and separate channel to the right) under the control of the 54H11-Gal4 driver labels numerous cells that cross from the CB towards the OL and associate with the lobula complex neuropil. Repo antibody (magenta, and separate channel to the right) is used to label glial cells. (E, F) UMAP showing the integration of the clusters we identified as lobula neuropil glia. Cells are colored by their developmental stage of origin in (E) and their adult identities in (F). Note that astrocyte-like clusters (Astrocytes_1/2/3/4) derive from the validated L3 lobula complex neuropil glia cluster (red arrow), whereas EG (Ensheathing_1/2) are only represented by cells from P15 onwards (dashed-line arrow). The code used to generate the graphs can be found at <a href="https://zenodo.org/records/21890456" target="_blank">https://zenodo.org/records/21890456</a>. (TIFF) 2026-09-28T17:57:37Z Image Figure 10.1371/journal.pbio.3004015.s002 https://figshare.com/articles/figure/_p_Cluster_identity_in_the_integrated_dataset_and_the_origin_of_lobula_neuropil_glia_p_/34017619 CC BY 4.0