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oai:figshare.com:article/34017616 2026-09-28T17:52:29Z category_12 category_13 category_15 category_272 category_734 category_61 category_64 category_128 category_133 portal_5 item_type_1 month_year_09_2026

Newly identified glia-specific genes. Amanda A. G. Ferreira (25132828) Sergio Córdoba (645937) Raghuvanshi Rajesh (25132831) Ben Jiwon Choi (25132834) Claude Desplan (45436) Cell Biology Genetics Neuroscience Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Developmental Biology Cancer Hematology Plant Biology innovative computational approach genetic markers expressed div >< p subcellular mrna compartmentalization optic lobe glia two large single detailed transcriptomic analysis identify mrna species glial diversity observed adult visual system glial cell bodies glial cell types understand glial development visual system cell mrna cell bodies glial types glial cells glia types adult stages cell class cell atlas neuropil glia chiasm glia work provides transcriptionally appear pupal stages helps explain gradually change drosophila </ distinct mrnas differentially localized developmental trajectories developmental single comprehensively annotated also present allowed us (A) Pearson correlation coefficient of larval clusters from Konstantinides and colleagues, 2022 with elav-positive bulk transcriptome (x-axis) and repo-positive transcriptome (y-axis) from Özel and colleagues, 2021. (A’) UMAP representation of all cells in the larval dataset from Konstantinides and colleagues, 2022. Glial cells are in red and non-glial cells in dark gray. (B) Table showing the top differentially expressed genes between Glial and Non-Glial cells. (C) Dot plot representing the expression of all genes from (B) by glial clusters in the larva dataset. (D) Combined HCR-RNA FISH and antibody staining for detection of both repo mRNA (cyan and separate channel below) and Repo protein (magenta and separate channel below). Note that surface glia presents a strong protein staining but weak mRNA signal. (E) Double staining of AnxB9 (cyan and separate channel to the right) and Repo (magenta) antibodies. (F) Double staining of CG32032 (cyan and separate channel to the right) and Repo (magenta) antibodies. (G) Dot plot showing all non-annotated clusters from Kurmangaliyev and colleagues, 2020—a late pupa (P96) Optic Lobe dataset. Arrows point to clusters with glial cells that were not previously identified as glia due to lack of repo expression. (H) Dot plot showing the three newly identified glial genes by cluster of a ventral nerve cord (VNC) dataset (Allen and colleagues, 2020). Arrows point to clusters that were not identified as glia by regular pan-glial markers that were later annotated as glia by specific cell type markers by the authors. Open arrowheads show clusters that express high levels of AnxB9 that are most likely not glia, see (I). (I) Cross section of a larval VNC stained for AnxB9 (cyan and separate channel to the right) and Repo (magenta). Note that some AnxB9+ cells in the cortex do not present Repo antibody (yellow arrowheads) and may be neurons. pg = perineurial glia, spg = subperineurial glia, eg = epithelial glia, mg = marginal glia, mng = medulla neuropil glia, xgi = inner chiasm glia and np = neuropil. The code used to generate the graphs can be found at <a href="https://zenodo.org/records/21890456" target="_blank">https://zenodo.org/records/21890456</a>. (TIFF) 2026-09-28T17:57:37Z Image Figure 10.1371/journal.pbio.3004015.s001 https://figshare.com/articles/figure/_p_Newly_identified_glia-specific_genes_p_/34017616 CC BY 4.0