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oai:figshare.com:article/34017577 2026-09-28T17:52:12Z category_12 category_24 category_272 category_39 category_734 category_69 category_106 category_132 category_134 portal_5 item_type_1 month_year_09_2026

Targeting SVCV G palmitoylation with a peptide inhibitor. Chen Li (54018) Yan Zhang (8098) Yujun Zhang (118792) Yan Gao (93649) Yuanan Lu (324035) Shengbo Cao (105106) Jing Ye (94291) Xueqin Liu (494581) Cell Biology Evolutionary Biology Environmental Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified Inorganic Chemistry Science Policy Infectious Diseases Virology significant economic impacts pathogenicity remain unclear modification enhances stability hold broader significance employed spring viremia competitive peptide targeting cell membrane localization 20 &# 176 molecular mechanisms underlying affects rhabdovirus infections temperature adaptive evolution rhabdovirus prevalent mechanism underlying xlink "> svcv glycoprotein svcv ), similar mechanism palmitic acid numerous rhabdoviruses high infectivity global aquaculture g protein g ). findings elucidate dependent infectivity crucial factor carp virus c ), aquatic rhabdoviruses acyltransferase zdhhc15a (A) Schematic of CPP-GS1 and CPP-GS2 peptides. The different residues are shown in red. CPPtat, CPP from HIV-1 Tat protein. (B) ZF4 cells were transfected with SVCV G for 6 h and then were incubated of CPPtat-GS1 and CPPtat-GS2 for 18 h. IP-ABE detection of palmitoylation of SVCV G in ZF4 cells. (C) ZF4 cells were transfected with SVCV G for 6 h and then were incubated of CPPtat-GS1 and CPPtat-GS2 for 18 h. Then, the cells were analyzed for SVCV G expression. (D-E) ZF4 cells were infected with SVCV G for 6 h and then were incubated of CPPtat-GS1 and CPPtat-GS2 for 18 h. Cell samples and supernatant were collected 24 h after infection. The viral RNA abundance was detected using qRT-PCR. SVCV titers were measured by Viral plaque assay. (F-G) ZF4 cells were infected with SVCV for 6 h and then were incubated with different concentrations of CPPtat-GS1 for 18 h. Cell samples and supernatant were collected 24 h after infection. The viral RNA abundance was detected using qRT-PCR. SVCV titers were measured by Viral plaque assay. (H) Zebrafish, with an average body weight of 0.6 g. At 6 h post-infection with 104 PFU SVCV, zebrafish were divided into distinct treatment groups (n = 30) and administered either DMSO or the CPP-GS1 peptide (50mg/kg). The survival rate of the zebrafish was subsequently assessed 14 days post-injection. Statistics of survival rate were calculated with the log-rank (Mantel-Cox). ***p < 0.001. (I) The virus titer of SVCV at 5 dpi from the spleen, intestine, and brain after CPP-GS1 administration (n = 3 zebrafish per group). Data was presented as mean ± SD from three independent experiments. Statistical analysis was performed using Student’s t-test and one-way ANOVA between different groups and the asterisk (*) indicates significant differences between groups*p < 0.05, **p < 0.01, ***p < 0.001. 2026-09-28T17:51:51Z Image Figure 10.1371/journal.ppat.1014654.g005 https://figshare.com/articles/figure/_p_Targeting_SVCV_G_palmitoylation_with_a_peptide_inhibitor_p_/34017577 CC BY 4.0