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oai:figshare.com:article/34017574 2026-09-28T17:52:11Z category_12 category_24 category_272 category_39 category_734 category_69 category_106 category_132 category_134 portal_5 item_type_1 month_year_09_2026

The G protein of low-temperature-sensitive rhabdovirus is palmitoylated. Chen Li (54018) Yan Zhang (8098) Yujun Zhang (118792) Yan Gao (93649) Yuanan Lu (324035) Shengbo Cao (105106) Jing Ye (94291) Xueqin Liu (494581) Cell Biology Evolutionary Biology Environmental Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified Inorganic Chemistry Science Policy Infectious Diseases Virology significant economic impacts pathogenicity remain unclear modification enhances stability hold broader significance employed spring viremia competitive peptide targeting cell membrane localization 20 &# 176 molecular mechanisms underlying affects rhabdovirus infections temperature adaptive evolution rhabdovirus prevalent mechanism underlying xlink "> svcv glycoprotein svcv ), similar mechanism palmitic acid numerous rhabdoviruses high infectivity global aquaculture g protein g ). findings elucidate dependent infectivity crucial factor carp virus c ), aquatic rhabdoviruses acyltransferase zdhhc15a (A) Protein palmitoylation software CSS-palm 4.0 was used to predict the VHSV G, IHNV G, SVCV G, MSRV G palmitoylation sites. (B) ZF4 cells were transfected with plasmids expressing VHSV G/WT and VHSV G(C/A) at 16 °C and subsequently mock treated (VHSV G/WT and VHSV G(C/A) groups) or treated with 50 μM 2-BP (VHSV G/WT 2-BP group). Protein palmitoylation was examined by IP-ABE assay in the presence and absence of HAM. The palmitoylated VHSV G and the immunoprecipitated VHSV G were blotted by HRP-streptavidin and His mAb, respectively. (C) ZF4 cells were transfected with plasmids expressing IHNV G/WT and IHNV G(C/A) at 16 °C and subsequently mock treated (IHNV G/WT and IHNV G(C/A) groups) or treated with 50 μM 2-BP (IHNV G/WT 2-BP group). Protein palmitoylation was examined by IP-ABE assay in the presence and absence of HAM. The palmitoylated IHNV G and the immunoprecipitated IHNV G were blotted by HRP-streptavidin and His mAb, respectively. (D-F) ZF4 cells were transfected with plasmids expressing VHSV G, IHNV G and MSRV G at 16 °C and 28 °C. Protein palmitoylation was examined by IP-ABE assay in the presence of HAM. The palmitoylated MSRV G, VHSV G and IHNV G and the immunoprecipitated VHSV G, IHNV G and MSRV G were blotted by HRP-streptavidin and His mAb, respectively. (G-H) ZF4 cells were transfected with the plasmid encoding VHSV G for 24h at 28 °C and 16 °C. Then, the cells were stimulated with CHX (1 µg/mL) and lysed at the indicated time points (0 h, 1 h, 2 h, and 4 h). The protein levels of VHSV G in the cell lysate were measured by Western blotting. (I-J) ZF4 cells were transfected with the plasmid encoding IHNV G for 24h at 28 °C and 16 °C. Then, the cells were stimulated with CHX (1 µg/mL) and lysed at the indicated time points (0 h, 1 h, 2 h, and 4 h). The protein levels of IHNV G in the cell lysate were measured by Western blotting. (K) ZF4 cells were transfected with the plasmid encoding MSRV G and were then treated with Orlistat (25µM, 50 µM, and 100 µM) or DMSO for 24 h at 16 °C. The MSRV G protein levels were measured by Western blotting. (L) ZF4 cells were transfected with the plasmid encoding MSRV G and were then treated with 2-BP (25µM, 50 µM, and 100 µM) or DMSO for 24 h at 16 °C. The MSRV G protein levels were measured by Western blotting. (M-N) ZF4 cells were transfected with the plasmid encoding VHSV G and IHNV G and were then treated with Orlistat (25µM, 50 µM, and 100 µM) or DMSO for 24 h at 16 °C. The VHSV G and IHNV G protein levels were measured by Western blotting. (O-P) ZF4 cells were transfected with the plasmid encoding VHSV G and IHNV G and were then treated with 2-BP (25µM, 50 µM, and 100 µM) or DMSO for 24 h at 16 °C. The VHSV G and IHNV G protein levels were measured by Western blotting. 2026-09-28T17:51:51Z Image Figure 10.1371/journal.ppat.1014654.g004 https://figshare.com/articles/figure/_p_The_G_protein_of_low-temperature-sensitive_i_rhabdovirus_i_is_palmitoylated_p_/34017574 CC BY 4.0