2026-10-14T03:24:36Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34017571 2026-09-28T17:52:10Z category_12 category_24 category_272 category_39 category_734 category_69 category_106 category_132 category_134 portal_5 item_type_1 month_year_09_2026

The low temperature-induced acyltransferase ZDHHC15a mediates palmitoylation of SVCVG. Chen Li (54018) Yan Zhang (8098) Yujun Zhang (118792) Yan Gao (93649) Yuanan Lu (324035) Shengbo Cao (105106) Jing Ye (94291) Xueqin Liu (494581) Cell Biology Evolutionary Biology Environmental Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified Inorganic Chemistry Science Policy Infectious Diseases Virology significant economic impacts pathogenicity remain unclear modification enhances stability hold broader significance employed spring viremia competitive peptide targeting cell membrane localization 20 &# 176 molecular mechanisms underlying affects rhabdovirus infections temperature adaptive evolution rhabdovirus prevalent mechanism underlying xlink "> svcv glycoprotein svcv ), similar mechanism palmitic acid numerous rhabdoviruses high infectivity global aquaculture g protein g ). findings elucidate dependent infectivity crucial factor carp virus c ), aquatic rhabdoviruses acyltransferase zdhhc15a (A) RNA interference effect on the expression of endogenous ZDHHC15a. ZF4 cells were transfected with ZDHHC15a-specific siRNA or control siRNA (NC) for 24 h. The efficiency of siRNAs to inhibit the ZDHHC15a expression was detected by qRT-PCR. (B) ZF4 cells were transfected with si-ZDHHC15a or si-NC for 12 h and then were transfected with the plasmid encoding SVCV G for 24 h. Protein palmitoylation was examined by IP-ABE assay. The palmitoylated SVCV G and the immunoprecipitated SVCV G were blotted by HRP-streptavidin and SVCV G mAb, respectively. (C) ZF4 cells were transfected with si-ZDHHC15a or si-NC for 12 h and then were transfected with the plasmid encoding SVCV G for 24 h. Then, the cells were analyzed for SVCV G expression. (D) ZF4 cells were infected with SVCV for 24 h. The supernatant of WCL was immunoprecipitated using anti-SVCV G magnetic beads magnetic beads. Then, the precipitated proteins were analyzed by Western blotting. (E-F) ZF4 cells were transfected with si-ZDHHC15a or si-NC for 12 h and then infected with SVCV(MOI = 10) for 24 h. Cell samples and supernatant were collected 24 h after infection. The viral RNA abundance was detected using qRT-PCR. SVCV titers were measured by Viral plaque assay. (G) Percentage survival of wild-type and zdhhc15a-/- zebrafish (n = 30) injected with 10 µl SVCV (107PFU/ml). Statistics of survival rate were calculated with the log-rank (Mantel-Cox). ***p < 0.001. (H) The virus titer in tissues were detected by virus titration in zebrafish. Data was presented as mean ± SD from three independent experiments. Statistical analysis was performed using Student’s t-test between different groups and the asterisk (*) indicates significant differences between groups*p < 0.05, **p < 0.01, ***p < 0.001. 2026-09-28T17:51:51Z Image Figure 10.1371/journal.ppat.1014654.g003 https://figshare.com/articles/figure/_p_The_low_temperature-induced_acyltransferase_ZDHHC15a_mediates_palmitoylation_of_SVCVG_p_/34017571 CC BY 4.0