2026-10-06T18:25:29Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34017203 2026-09-28T17:43:05Z category_1 category_4 category_7 category_8 category_12 category_13 category_14 category_16 category_21 category_24 category_873 category_734 category_61 category_64 category_132 category_133 category_135 portal_5 item_type_3 month_year_09_2026

<p>List of RT-qPCR primers.</p> Hsiao-Yuh Roan (25132784) Xuejiao Tian (20523980) Wei-Chen Chu (450265) Chia-Ming Lee (12987725) Hsin Chen (2365045) Fiorency Santoso (25132787) Chung-Han Wang (19334639) Yu-Hsiu Liu (4254004) Uday Kumar (16457565) He-Yun Hsiao (25132790) Chiou-Hwa Yuh (253039) Bi-Chang Chen (2656468) Chen-Hui Chen (739721) Biophysics Biochemistry Medicine Microbiology Cell Biology Genetics Molecular Biology Physiology Biotechnology Evolutionary Biology Chemical Sciences not elsewhere classified Biological Sciences not elsewhere classified Developmental Biology Cancer Infectious Diseases Plant Biology Computational Biology using transgenic reporters temporally concentrated burst overall body growth monitor individual cells monitor diseased livers linear growth regimen label hepatocyte nuclei findings bridge micrometer every hepatocyte within 5 mm thickness cell number increases cell number increased organ expansion microscopy scale cell behaviors embryonic growth period bona fide single scale organ growth adult vertebrate organs also utilized wao embryonic period organ scale adult organs cell resolution cell proliferation organ single wide process toto single tissue architecture resolved visualization lineage tracing integrated workflow including heart generalizable platform fully resolved clonal organization 206 hepatocytes <div><p>Vertebrate organs undergo massive growth during the post-embryonic period. Yet, our understanding of how this organ-wide process is organized at single-cell resolution has been limited by an inability to monitor individual cells of different types throughout intact adult organs. Here we establish an integrated workflow of whole adult-organ expansion microscopy (WAO-ExM) that enables in toto single-cell-resolved visualization of every hepatocyte within a complete adult vertebrate liver. Using transgenic reporters to label hepatocyte nuclei, we quantified cell expansion dynamics across the entire post-embryonic growth period, finding that an intact adult liver spanning ~5 mm thickness had an average of 1,265,206 hepatocytes. The data further revealed a non-linear growth regimen in which cell number increased by 538-fold, with a temporally concentrated burst that was not reflective of overall body growth. Integration of lineage tracing with WAO-ExM revealed that cell number increases were driven by a few hepatocytes undergoing drastic clonal expansion at the whole-organ scale. Disruption of extracellular matrix laminins decoupled liver shaping from total cell number increases, suggesting independent regulatory control of tissue architecture and cell proliferation. We also utilized WAO-ExM to monitor diseased livers and other adult organs, including heart and pancreas. Altogether, these findings bridge micrometer-scale cell behaviors with centimeter-scale organ growth, and establish a generalizable platform for adult vertebrate organs to be fully resolved at bona fide single-cell resolution.</p></div> 2026-09-28T18:01:19Z Dataset Dataset 10.1371/journal.pbio.3004007.s016 https://figshare.com/articles/dataset/_p_List_of_RT-qPCR_primers_p_/34017203 CC BY 4.0