2026-10-07T21:44:56Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34015125 2026-09-28T15:32:29Z category_24295 category_24784 item_type_3 month_year_09_2026

CyTOF analysis for A549 cell lines (4 LOY and 4 ROY) Michael Scherer (12212429) Kathleen Schlüter (23156971) Mei-Ju Chen (17477892) Maria Llamazares-Prada (21632297) Christoph Plass (14946945) Epigenetics (incl. genome methylation and epigenomics) Cancer genetics CyTOF histone modifications epigenomics and epigenetics <p dir="ltr">CyTOF data underwent the following pre-processing prior to analyses: First, the CyTOF software by Fluidigm was used for normalization and concatenation of the acquired data. Then, several gates were applied using the FlowJo platform:</p><p dir="ltr">Live single cells were gated using the cisplatin 195Pt and iridium DNA label in 191Ir. Next, the normalization beads were gated out using the 140Ce channel. Then, event length, and the Gaussian parameters of width, center, offset and residual channels were used for normalization. Single Cell Debarcoder was used for samples de-barcoding.</p> 2026-09-28T15:32:29Z Dataset Dataset 10.6084/m9.figshare.34015125.v1 https://figshare.com/articles/dataset/CyTOF_analysis_for_A549_cell_lines_4_LOY_and_4_ROY_/34015125 CC BY 4.0