2026-10-13T11:41:54Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34014609 2026-09-28T14:08:04Z category_19 portal_316 item_type_3 month_year_09_2026

Data Sheet 1_Extracellular vesicle-mediated preconditioning of osteoblasts by ER+ breast cancer cells: implications for the bone metastatic niche.pdf De Shanna M. Johnson (25122924) Edward A. Shangin (25122927) Hannah L. Marrone (25122930) Elisabeth J. Richardson (25122933) Danielle O. Barkers-Woode (25122936) Caitlyn J. Collins (13030521) L. Monet Roberts (25122939) Pharmacology breast-to-bone metastasis estrogen receptor positive (ER+) extracellular vesicles intercellular communication molecular mechanisms <p>Breast cancer metastasis to bone most commonly occurs in late-stage disease and remains a major contributor to morbidity, reduced quality of life, and mortality, particularly in estrogen receptor-positive (ER+) tumors. Intercellular communication between tumor cells and bone-resident cells plays a critical role in promoting tumor invasion and development of metastatic bone lesions. Historically, tumor-bone interactions have been viewed largely through the lens of soluble factor-mediated signaling, particularly in the context of osteoclast-driven bone resorption and osteolytic lesion formation. However, the majority of bone metastases exhibit a mixed lytic and sclerotic phenotype, reflecting the combined involvement of osteoclast- and osteoblast-mediated remodeling. Here, we investigate how ER+ breast cancer-derived extracellular vesicle-enriched conditioned media influence osteoblast behavior and signaling in vitro, positioning osteoblasts as key intermediaries in bone metastatic progression. We first show that ER+ T47D cells release significantly higher EVs than MCF10A non-tumorigenic breast epithelial cells. Next, we demonstrate that osteoblasts actively internalize T47D-derived EVs by 24 h, resulting in significant alterations in their proliferation and signaling profiles associated with chemotaxis and vascular-associated pathways. Finally, we show that conditioned media from T47D EV-preconditioned osteoblasts did not significantly enhance breast tumor cell proliferation or overall motility outcomes. However, despite similar mean motility metrics between conditions, migration behavior exhibited distinct population-level distributions. Collectively, these findings demonstrate that ER+ breast cancer-derived EVs drive selective reprogramming of osteoblast signaling and function as an important component in tumor-bone niche conditioning.</p> 2026-09-28T14:08:04Z Dataset Dataset 10.3389/fphar.2026.1844422.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Extracellular_vesicle-mediated_preconditioning_of_osteoblasts_by_ER_breast_cancer_cells_implications_for_the_bone_metastatic_niche_pdf/34014609 CC BY 4.0