2026-10-14T09:46:01Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34013767 2026-09-28T12:35:26Z category_4 category_7 category_12 category_15 category_16 category_873 category_46 category_734 category_64 category_122 portal_87 item_type_3 month_year_09_2026

Neuroprotective Effects of <i>Nanocurcumin</i> in Spinal Cord Injury: Modulation of Inflammatory and Inflammasome-Associated Signaling in an Integrated <i>In Vivo</i>, <i>In Silico</i>, and Machine-Learning Study Seyyedeh Fahimeh Talebi (25122415) Jalal Mardaneh (13744039) Mohammad Matin Alishani (19434487) Abeer Ali Kadhim (25122418) Pasha Mosaed (25122421) Ako Mohammadi (25122424) Sajad Najafi (8523714) Jamal Majidpoor (18301825) Hamed Shoorei (10272620) Biochemistry Medicine Cell Biology Neuroscience Physiology Chemical Sciences not elsewhere classified Immunology Biological Sciences not elsewhere classified Cancer Mental Health Spinal Cord Injury Nanocurcumin Inflammasome Inflammation In Silico Machine-Learning <p>Spinal cord injury (SCI) triggers secondary neuroinflammatory and inflammasome-associated responses that contribute to tissue damage and functional impairment. This study evaluated <i>nanocurcumin</i> in experimental SCI using integrated experimental, computational, and exploratory machine-learning approaches. Adult male Wistar rats were allocated to Sham, SCI, or SCI + <i>nanocurcumin</i> groups. SCI was induced by T6 contusion, and <i>nanocurcumin</i> (50 mg/kg, intraperitoneally) was administered every 12 hours for 72 hours. Functional, histological, molecular, and inflammatory outcomes were assessed using BBB scoring, Luxol Fast Blue staining, immunofluorescence, RT-qPCR, and ELISA. SCI impaired locomotor function and myelin integrity and increased inflammasome-associated and inflammatory markers. <i>Nanocurcumin</i> significantly improved BBB scores, increased the LFB-positive myelinated area, and attenuated these molecular alterations. Tissue IL-1β, IL-18, HMGB1, and TLR4 levels were also reduced following <i>nanocurcumin</i> treatment compared with untreated SCI. Correlation and hierarchical clustering analyses demonstrated coordinated molecular–functional associations and separation of SCI from control and treated profiles. Exploratory Random Forest/SHAP analyses highlighted NF-κB, Caspase-1, NOX2, and NLRP3 as influential variables for group discrimination. Docking predicted favorable curcumin–target interactions, while MD simulations supported distinct conformational behavior of selected complexes. Overall, <i>nanocurcumin</i> was associated with attenuated inflammatory and inflammasome-associated responses and improved early functional and histological outcomes following SCI.</p> 2026-09-28T12:35:26Z Dataset Dataset 10.6084/m9.figshare.34013767.v1 https://figshare.com/articles/dataset/Neuroprotective_Effects_of_i_Nanocurcumin_i_in_Spinal_Cord_Injury_Modulation_of_Inflammatory_and_Inflammasome-Associated_Signaling_in_an_Integrated_i_In_Vivo_i_i_In_Silico_i_and_Machine-Learning_Study/34013767 CC BY 4.0