2026-10-06T14:09:46Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34009560 2026-09-28T05:44:46Z category_1293 portal_316 item_type_3 month_year_09_2026

Data Sheet 1_Identification of pathogens causing mulberry leaf spot and resistance evaluation of mulberry germplasms in Hechi, China.pdf Shihao Zhang (5165825) Jiping Liu (217497) Kangdong Huang (25120116) Liuying Wen (828354) Lingling Pan (548437) Xiumei Huang (10204692) Weiwei Wu (244139) Yue Qin (634751) Xiaodong Li (181281) Agronomy leaf spot mulberry pathogen identification resistance resource SSR Introduction<p>Mulberry leaf spot, caused by fungal pathogens, is an economically devastating disease that leads to severe yield losses and reduces the nutritional quality of foliage, ultimately impairing the growth and development of silkworms. However, the causal agents of this disease in Hechi remain largely unidentified, and the availability of resistant mulberry cultivars is equally unclear.</p>Methods<p>In this study, pathogens were isolated from symptomatic mulberry samples, and isolated strains were identified based on morphological characterization and molecular analyses of the ITS, TUB2, and ACT sequences. Following Koch’s postulates, the virulent isolates were recognized. Accordingly, three highly pathogenic fungal strains were selected as target pathogens and used to evaluate the disease resistance of 28 mulberry varieties by artificial inoculation of detached leaves with these pathogens. A set of 20 simple sequence repeat (SSR) markers was used to analyze 28 mulberry varieties.</p>Results<p>Our findings reveal that the causal agents of mulberry leaf spot in Hechi are Diaporthe tulliensis (SY53), Corynespora cassiicola (SY123), and Paramythecium foliicola (SY151), with D. tulliensis and C. cassiicola being new records worldwide. Among the 28 varieties tested, Gui 12, Gui 5, Wuguoganda, Nongsang 14, Jialing 30, Zhongsang 9703, and Hubeizijing exhibited relatively high resistance to all three strains. Furthermore, a dendrogram based on the 20 SSR marker set grouped the 28 varieties into four clusters, which separated resistant accessions from susceptible ones.</p>Discussion<p>This study identified the pathogens responsible for mulberry leaf spot in Hechi and screened disease-resistant mulberry varieties, providing a solid basis for the discovery of resistance genes and molecular breeding of mulberry.</p> 2026-09-28T05:44:46Z Dataset Dataset 10.3389/fagro.2026.1930317.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Identification_of_pathogens_causing_mulberry_leaf_spot_and_resistance_evaluation_of_mulberry_germplasms_in_Hechi_China_pdf/34009560 CC BY 4.0