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oai:figshare.com:article/34008381 2026-09-28T04:30:13Z category_660 portal_316 item_type_3 month_year_09_2026

Supplementary file 1_Divergent cellular responses to sciatic nerve crush versus transection: bilateral MYCN induction and distinct DRG neuronal responses.docx Shilian Zhou (25119468) Ziyan Huang (3863497) Zhongyuan Lin (651801) Jingwei Chen (1578331) Xinyu Zhang (14029) Diya Wang (5018522) Haihua Shu (12019018) Fang Ye (112854) Cellular Interactions (incl. Adhesion, Matrix, Cell Wall) ATF3 bilateral spinal response MYCN nerve regeneration peripheral nerve injury sciatic nerve axotomy sciatic nerve crush sensory dysfunction Introduction<p>Peripheral nerve injuries can produce regenerative or persistent-disconnection outcomes depending on the injury modality, but the underlying cellular responses and their effects on the central nervous system remain incompletely defined.</p>Methods<p>We compared sciatic nerve crush (SNC; axonotmesis) and sciatic nerve transection (SNT; neurotmesis) in wild-type, AdvCre;Ai14, and MYCN-eGFP reporter mice. Longitudinal assessments included functional recovery, neuronal injury responses marked by ATF3, IB4-positive sensory-neuron responses, dorsal-horn microglial activation, and spinal MYCN-eGFP signal.</p>Results<p>SNC was associated with partial, measure-specific functional recovery, a transient and self-limited ATF3 response, and a limited reduction in IB4-positive neuronal profiles. In contrast, SNT produced persistent axotomy-associated neuronal stress, a reduction in IB4-positive neuronal profiles, and more protracted dorsal-horn microglial activation. MYCN-eGFP intensity was significantly increased above sham levels bilaterally in spinal ventral-horn neurons after both SNC and SNT at postoperative day 7 (POD7), with the greatest acute amplitude observed after SNC. From POD7 to POD14, MYCN-eGFP intensity declined by more than 90% after SNC but by approximately 60% after SNT, indicating distinct temporal patterns between the two injury modalities.</p>Discussion<p>SNC and SNT engage overlapping but quantitatively and temporally distinct cellular programs, including modality-specific dorsal root ganglion neuronal responses associated with functional recovery. These findings refine the interpretation of two widely used peripheral nerve injury models and identify bilateral spinal MYCN-eGFP dynamics as a candidate cellular readout for future mechanistic investigation.</p> 2026-09-28T04:30:13Z Dataset Dataset 10.3389/fncel.2026.1922426.s001 https://figshare.com/articles/dataset/Supplementary_file_1_Divergent_cellular_responses_to_sciatic_nerve_crush_versus_transection_bilateral_MYCN_induction_and_distinct_DRG_neuronal_responses_docx/34008381 CC BY 4.0