2026-10-09T18:41:21Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34007760 2026-09-30T00:28:31Z category_24139 portal_12 item_type_5 month_year_09_2026

Yuan Wang: Development of Plant-Based 3D Printed Scaffolds for Bovine Satellite Cell Maturation in Cell-Based Meat Production Yuan Wang (17062137) Jake Oh (4074280) Ang Jin (21357651) Renwick Dobson (4089007) Olaf Diegel (6202220) Jenny Malmström (2477113) Laura Domigan (1202520) Cell development, proliferation and death plant protein isolate bioink extrusion bioprinting high-fidelity printing cultured meat scaffold stability <p dir="ltr">Cell-based meat production requires edible scaffolds that provide structural support for muscle cell attachment, growth, and tissue formation. This study developed a 3D-printed plant-protein scaffold for bovine satellite cell culture. Plant-protein inks were characterized for flow behaviour and extrusion printability, followed by fabrication of lattice scaffolds and assessment of their stability in DPBS. The inks exhibited shear-thinning behaviour and temperature-dependent viscosity, enabling extrusion-based 3D printing. Printed lattices retained their visible structure during seven days of incubation in DPBS. Bovine satellite cells attached to the scaffolds and displayed F-actin organization, while Live/Dead staining indicated the presence of viable cells. PrestoBlue measurements further showed increasing metabolic activity over seven days of culture. These results demonstrate that 3D-printed plant-protein scaffolds can provide a stable, food-compatible platform supporting early bovine muscle cell growth, with future work focusing on myogenic differentiation and maturation.</p> 2026-09-30T00:28:31Z Image Poster 10.17608/k6.auckland.34007760.v2 https://figshare.com/articles/poster/Development_of_Plant-Based_3D_Printed_Scaffolds_for_Bovine_Satellite_Cell_Maturation_in_Cell-Based_Meat_Production/34007760 CC BY 4.0