2026-10-12T01:35:15Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34004538 2026-09-30T00:30:58Z category_24478 portal_12 item_type_5 month_year_09_2026

Du Young Kim: Long-term memory impairment following general anaesthesia varies with circadian time of exposure in Drosophila melanogaster Du Young Kim (8147058) Guy Warman (1193709) David Cumin (1188780) James Cheeseman (1196307) Anaesthesiology general anaesthesia circadian clock long-term memory isoflurane Drosophila melanogaster PERIOD <p dir="ltr">General anaesthesia can impair memory and shift the circadian clock, but whether circadian timing influences the severity of this impairment remains unknown. We tested this in Drosophila melanogaster using a 24-hour appetitive memory assay. Flies were trained to associate an odour with a sugar reward, then exposed to 2% isoflurane for 3 hours at one of 17 circadian timepoints. Isoflurane impaired long-term memory at every exposure time compared with untreated controls. Severity varied across the cycle, with performance lowest around circadian time (CT) 9.5 to 10.5 and least impaired at CT 22.5. The low point aligns with expected low abundance of PERIOD, a core circadian clock protein, while CT 22.5 aligns with expected high abundance. Whether PERIOD accounts for the timing effect is not yet established. Ongoing work tests whether isoflurane disrupts molecular clock rhythms and locomotor behaviour at the same circadian times, and whether timed light alters these effects.</p> 2026-09-30T00:30:58Z Image Poster 10.17608/k6.auckland.34004538.v2 https://figshare.com/articles/poster/Long-term_memory_impairment_following_general_anaesthesia_varies_with_circadian_time_of_exposure_in_Drosophila_melanogaster/34004538 CC BY 4.0