2026-10-14T05:12:53Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34003941 2026-09-26T19:52:44Z category_24367 category_24388 category_23884 item_type_2 month_year_09_2026

<b><i>Citrus tristeza virus</i></b><b> (CTV): 3D Virion Architecture, Vector Transmission Biophysics (EPG), and Field Diagnostics</b> Lalit Patil (23013781) Pratiksha Biradar (25115058) Shaivya Singh (25115059) Halima Khatoon (25115061) Bharat Raj Meena (25115066) Elangovan Marimuthu (25115068) Ngawang Namgey (25115069) Virology Plant pathology Agriculture, land and farm management not elsewhere classified Citrus tristeza virus Closteroviridae Toxoptera citricida Aphid EPG biophysics RT-LAMP diagnostics Stem pitting Graft transmission <p dir="ltr">This audiovisual documentary and scientific visualization covers the structural biology, transmission dynamics, and field diagnostics of <i>Citrus tristeza virus </i>(CTV, genus <i>Closterovirus</i>).</p><p dir="ltr"><b>Key Modules Featured:</b></p><ol><li><b>Virion Architecture</b>: 2000 nm flexuous rod showcasing the major capsid protein (CP/p25) covering ~95% of the genome and the minor capsid protein (CPm/p27) forming the 5' rattle snake-like terminal structure.</li><li><b>Comparative Genomics</b>: Alignment track analyzing divergence across virulent quick-decline (VT), severe stem-pitting (T36), mild cross-protecting (T30), and Indian acid lime (Kpg) isolates in p33, p20, and 5' UTR regions.</li><li><b>Vector Transmission Biophysics</b>: Real-time Electrical Penetration Graph (DC-EPG) waveforms representing intercellular pathway (waveform C), intracellular potential drops (pd), phloem salivation/inoculation (E1), and phloem sap ingestion/acquisition (E2) by the brown citrus aphid (<i>Toxoptera citricida</i>).</li><li><b>Rootstock Incompatibility Risk</b>: Cambial necrosis analysis comparing Sweet Orange on Sour Orange rootstocks against tolerant Rough Lemon and Rangpur Lime cultivars.</li><li><b>Rapid Field Diagnostics</b>: Conserved CP/p25 isothermal amplification (RT-LAMP) at 65°C with colorimetric visual dye indicators (HNB and Phenol Red) coupled with lateral flow dipstick chromatography.</li></ol><p></p> 2026-09-26T19:52:44Z Dataset Media 10.6084/m9.figshare.34003941.v1 https://figshare.com/articles/media/_b_i_Citrus_tristeza_virus_i_b_b_CTV_3D_Virion_Architecture_Vector_Transmission_Biophysics_EPG_and_Field_Diagnostics_b_/34003941 MIT