2026-10-12T18:12:35Z https://api.figshare.com/v2/oai

oai:figshare.com:article/34003665 2026-09-26T16:09:13Z category_4 category_7 category_21 category_873 category_46 category_61 category_134 category_135 portal_63 item_type_2 month_year_09_2026

Nanofibrous Poly(amino acid ester) Dressing for Promoting Diabetic Wound Healing via Spatiotemporal Coordination of Immunomodulation and Tissue Regeneration Li Ma (46633) Ying Feng (151159) Kaijun Li (634814) Yangrui Du (14801251) Hexin Zhou (24035742) Ying Ji (285782) Kaikai Ma (3116910) Tao Shuai (25114836) Gongyan Liu (6170081) Biochemistry Medicine Biotechnology Chemical Sciences not elsewhere classified Immunology Developmental Biology Virology Computational Biology timely proinflammatory response spontaneous shift toward held electrospinning using amino acid ester allowing sustained release accelerated wound closure spatiotemporal metabolic modulation diabetic wound microenvironment rationally designed arginine breathable nanofibrous dressing wound epidermis spatiotemporal coordination nanofibrous poly diabetic wounds work offers tissue regeneration thereby restoring spatially promoting situ </ responsive degradation reparative state promising strategy progressive deterioration often overlooked mimicking dressing initially promoting extracellular matrix even led epidermal keratinocytes conventional treatments containing degradants commercial dressings based poly 21 days 14 days Effective healing of diabetic wounds requires spatiotemporal coordination of immunoregulation and tissue regeneration, which is often overlooked in conventional treatments. Here, we developed an extracellular matrix-mimicking dressing, termed ANM, that modulates arginine utilization within the diabetic wound microenvironment to re-establish the spatiotemporal coordination of healing. ANM was <i>in situ</i> fabricated by hand-held electrospinning using a rationally designed arginine- and norvaline-based poly(ester amide), yielding a conformable and breathable nanofibrous dressing. Its porous and hydrolyzable structure enabled moisture- and enzyme-responsive degradation, allowing sustained release of arginine- and norvaline-containing degradants. These bioactive degradants cooperatively modulated macrophage phenotypic transitions, initially promoting a timely proinflammatory response, followed by a spontaneous shift toward a pro-reparative state, thereby restoring the temporally sequenced inflammatory program in diabetic wounds. Meanwhile, ANM locally upregulated inducible nitric oxide synthase (iNOS) and arginase-1 in the peri-wound epidermis, spatially promoting the proliferation and differentiation of epidermal keratinocytes and accelerating re-epithelialization. In contrast to commercial dressings that failed to support healing and even led to progressive deterioration over 21 days, ANM significantly promoted collagen deposition and angiogenesis and accelerated wound closure in db/db mice by 14 days. This work offers a promising strategy for treating chronic nonhealing wounds through spatiotemporal metabolic modulation. 2026-09-26T00:00:00Z Dataset Media 10.1021/acsnano.6c07848.s002 https://figshare.com/articles/media/Nanofibrous_Poly_amino_acid_ester_Dressing_for_Promoting_Diabetic_Wound_Healing_via_Spatiotemporal_Coordination_of_Immunomodulation_and_Tissue_Regeneration/34003665 CC BY-NC 4.0